ArticleInvestigative ophthalmology & visual science2013
Effect of growth factors on the proliferation and gene expression of human meibomian gland epithelial cells.
Article in Investigative ophthalmology & visual science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
- Identification of Meibomian gland stem cell populations and mechanisms of aging.Nature communications · 2025Article
- Identification of Meibomian gland stem cell populations and mechanisms of aging.bioRxiv : the preprint server for biology · 2024Article
- Expression of ATP-Binding Cassette Transporter A1 (ABCA1) in Eyelid Tissues and Meibomian Gland Epithelial Cells.Investigative ophthalmology & visual science · 2024Article
- A novel co-culture model of human prostate epithelial and stromal cells for androgenic and antiandrogenic screening.Toxicology in vitro : an international journal published in association with BIBRA · 2023Article
- Expression of Acyl-CoA wax-alcohol acyltransferase 2 (AWAT2) by human and rabbit meibomian glands and meibocytes.The ocular surface · 2022Article
- Triacylglycerol lipidome from human meibomian gland epithelial cells: Description, response to culture conditions, and perspective on function.Experimental eye research · 2021Article
- Effects of nanopatterned-surface dishes on chondrocyte growth and cell cycle progression.RSC advances · 2020Article
- Article
- The Role of Hypoxia-Inducible Factor 1α in the Regulation of Human Meibomian Gland Epithelial Cells.Investigative ophthalmology & visual science · 2020Article
- Hypoxia: A breath of fresh air for the meibomian gland.The ocular surface · 2019Article
- Biomarkers for Progenitor and Differentiated Epithelial Cells in the Human Meibomian Gland.Stem cells translational medicine · 2018Article
- PPARγ regulates meibocyte differentiation and lipid synthesis of cultured human meibomian gland epithelial cells (hMGEC).The ocular surface · 2018Article
- The Effect of Solithromycin, a Cationic Amphiphilic Drug, on the Proliferation and Differentiation of Human Meibomian Gland Epithelial Cells.Current eye research · 2018Article
- Meibomian glands, meibum, and meibogenesis.Experimental eye research · 2017Review
- PPARγ Regulates Mouse Meibocyte Differentiation and Lipid Synthesis.The ocular surface · 2016Article
- Do Cyclosporine A, an IL-1 Receptor Antagonist, Uridine Triphosphate, Rebamipide, and/or Bimatoprost Regulate Human Meibomian Gland Epithelial Cells?Investigative ophthalmology & visual science · 2016Article
- Article
- Dissecting lipid metabolism in meibomian glands of humans and mice: An integrative study reveals a network of metabolic reactions not duplicated in other tissues.Biochimica et biophysica acta · 2016Article
- Effects of Insulin and High Glucose on Human Meibomian Gland Epithelial Cells.Investigative ophthalmology & visual science · 2015Article
- Serum-induced keratinization processes in an immortalized human meibomian gland epithelial cell line.PloS one · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
purposeWe hypothesize that growth factors, including epidermal growth factor (EGF) and bovine pituitary extract (BPE), induce proliferation, but not differentiation (e.g., lipid accumulation), of human meibomian gland epithelial cells. We also hypothesize that these actions involve a significant upregulation of genes linked to cell cycle processes, and a significant downregulation of genes associated with differentiation. Our objective was to test these hypotheses.
methodsImmortalized human meibomian gland and conjunctival epithelial cells were cultured for varying time periods in the presence or absence of EGF, BPE, EGF + BPE, or serum, followed by cell counting, neutral lipid staining, or RNA isolation for molecular biological procedures.
resultsOur studies show that growth factors stimulate a significant, time-dependent proliferation of human meibomian gland epithelial cells. These effects are associated with a significant upregulation of genes linked to cell cycle, DNA replication, ribosomes, and translation, and a significant decrease in those related to cell differentiation, tissue development, lipid metabolic processes, and peroxisome proliferator-activated receptor signaling. Serum-induced differentiation, but not growth factor-related proliferation, elicits a pronounced lipid accumulation in human meibomian gland epithelial cells. This lipogenic response is unique, and is not duplicated by human conjunctival epithelial cells.
conclusionsOur results demonstrate that EGF and BPE stimulate human meibomian gland epithelial cells to proliferate. Further, our findings show that action is associated with an upregulation of cell cycle and translation ontologies, and a downregulation of genetic pathways linked to differentiation and lipid biosynthesis.
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