ArticleNature protocols2013
Microfluidic trap array for massively parallel imaging of Drosophila embryos.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 61 citations in OpenAlex.
- Current and Future Cornea Chip Models for Advancing Ophthalmic Research and Therapeutics.Advanced biology · 2025Review
- Cell pairing for biological analysis in microfluidic devices.Biomicrofluidics · 2022Review
- A robot-assisted acoustofluidic end effector.Nature communications · 2022Article
- Microfluidics for understanding model organisms.Nature communications · 2022Review
- An open-source semi-automated robotics pipeline for embryo immunohistochemistry.Scientific reports · 2021Article
- A polymer index-matched to water enables diverse applications in fluorescence microscopy.Lab on a chip · 2021Article
- High-Temporal-Resolution smFISH Method for Gene Expression Studies inAnalytical chemistry · 2021Article
- Bioelectronics for Millimeter-Sized Model Organisms.iScience · 2020Review
- A Method for Developing Novel 3D Cornea-on-a-Chip Using Primary Murine Corneal Epithelial and Endothelial Cells.Frontiers in pharmacology · 2020Article
- Integrating Microfabrication into Biological Investigations: the Benefits of Interdisciplinarity.Micromachines · 2019Article
- Design of a comprehensive microfluidic and microscopic toolbox for the ultra-wide spatio-temporal study of plant protoplasts development and physiology.Plant methods · 2019Article
- Tools to reverse-engineer multicellular systems: case studies using the fruit fly.Journal of biological engineering · 2019Review
- Microfluidic Based Optical Microscopes on Chip.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2018Review
- Microfabrication of AngioChip, a biodegradable polymer scaffold with microfluidic vasculature.Nature protocols · 2018Article
- Microfluidics for mechanobiology of model organisms.Methods in cell biology · 2018Article
- A microfluidic trap array for longitudinal monitoring and multi-modal phenotypic analysis of individual stem cell aggregates.Lab on a chip · 2017Article
- Synthesizing developmental trajectories.PLoS computational biology · 2017Article
- Microfluidic platform for efficient Nanodisc assembly, membrane protein incorporation, and purification.Lab on a chip · 2017Article
- Parallel imaging ofDisease models & mechanisms · 2017Article
- Characterization of microfluidic clamps for immobilizing and imaging ofBiomicrofluidics · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Here we describe a protocol for the fabrication and use of a microfluidic device to rapidly orient >700 Drosophila embryos in parallel for end-on imaging. The protocol describes master microfabrication (∼1 d), polydimethylsiloxane molding (few hours), system setup and device operation (few minutes) and imaging (depending on application). Our microfluidics-based approach described here is one of the first to facilitate rapid orientation for end-on imaging, and it is a major breakthrough for quantitative studies on Drosophila embryogenesis. The operating principle of the embryo trap is based on passive hydrodynamics, and it does not require direct manipulation of embryos by the user; biologists following the protocol should be able to repeat these procedures. The compact design and fabrication materials used allow the device to be used with traditional microscopy setups and do not require specialized fixtures. Furthermore, with slight modification, this array can be applied to the handling of other model organisms and oblong objects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.