Evidence map›Paper›PMID 23493053›Full record

ArticleIranian journal of basic medical sciences2012

Simultaneous Detection of Integrase and Antibiotic Resistance Genes within SXT Constin in Vibrio cholerae O1 El Tor Strains Isolated from Iran Using Multiplex-PCR.

Seyed Mahmoud Amin Marashi, Bahram Nasr Esfahani, Akbar Tavakoli, Sharareh Moghim, Mohammad Reza Pourshafie, Mansoor Salehi

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Article in Iranian journal of basic medical sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

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5citing papers in PubMed
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3 · Its place in the literature

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5 citing papers in PubMed.

  1. Review
  2. Resistance ofFrontiers in pharmacology · 2023
    Review
  3. Article
  4. Genetic Characterization of AmpC and Extended-Spectrum Beta-Lactamase Phenotypes inFrontiers in cellular and infection microbiology · 2021
    Article
  5. Article
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5 · Who and what money

Authors and funding

6 authors.

Seyed Mahmoud Amin MarashiDepartment of Microbiology & Immunology, Babol University of Medical Sciences, Babol, Iran.
Bahram Nasr Esfahani
Akbar Tavakoli
Sharareh Moghim
Mohammad Reza Pourshafie
Mansoor Salehi

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesAmongst the various antibiotic resistant elements in Vibrio. cholerae, SXT constin (SXT-C) is important. We were going to design a quick method for determination of antibiotic resistance gene pattern in SXT-C. MATERIALS AND

methodsNinety four V. cholerae O1 El Tor isolates were used in this study. Antibiotic susceptibility testing, multiplex PCR amplification of SXT-C containing dfrA1, sulII, strB and the int in a multiplex PCR were done.

resultsResults of our study showed that 92 (97.8%) out of 94 isolates were positive for all above genes. Multiplex PCR results correlated with the antibiotic susceptibility data obtained by using disc diffusion assay.

conclusionUsing this multiplex PCR, it would be easily possible to demonstrate the presence of antibiotic resistance in SXT-C which, in turn, allows for a suitable treatment in cholera patients causing reduction in the mortality and morbidity rate of the infected individuals.

Indexed as

Antibiotic resistance patternIntegraseMultiplex-PCRSXT constinVibrio cholerae

Identifiers

PMID23493053
PMCPMC3586891

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