ArticleProceedings of the National Academy of Sciences of the United States of America1990
Regulatory elements in the introns of the human HPRT gene are necessary for its expression in embryonic stem cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 102 citations in OpenAlex.
- RAD51 separation of function mutation disables replication fork maintenance but preserves DSB repair.iScience · 2024Article
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- CRISPR/Cas9-Mediated Scanning for Regulatory Elements Required for HPRT1 Expression via Thousands of Large, Programmed Genomic Deletions.American journal of human genetics · 2017Article
- Two replication fork maintenance pathways fuse inverted repeats to rearrange chromosomes.Nature · 2013Article
- RAD51 mutants cause replication defects and chromosomal instability.Molecular and cellular biology · 2012Article
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- delta-EF1 is a negative regulator of Ihh in the developing growth plate.The Journal of cell biology · 2009Article
- The word landscape of the non-coding segments of the Arabidopsis thaliana genome.BMC genomics · 2009Article
- TREX2 exonuclease defective cells exhibit double-strand breaks and chromosomal fragments but not Robertsonian translocations.Mutation research · 2009Article
- A viable mouse model of factor X deficiency provides evidence for maternal transfer of factor X.Journal of thrombosis and haemostasis : JTH · 2008Article
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- Prothrombin deficiency results in embryonic and neonatal lethality in mice.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Biological effects of targeted inactivation of hepatocyte growth factor-like protein in mice.The Journal of clinical investigation · 1998Article
- Elements in the distal 5'-flanking sequence and the first intron function cooperatively to regulate glutamine synthetase transcription during adipocyte differentiation.Nucleic acids research · 1997Article
- Stability of HPRT marker gene expression at different gene-targeted loci: observing and overcoming a position effect.Nucleic acids research · 1997Article
- Introduction of precise alterations into the mouse genome with high efficiency by stable tag-exchange gene targeting: implications for gene targeting in ES cells.Nucleic acids research · 1997Article
- Rescue of cardiac alpha-actin-deficient mice by enteric smooth muscle gamma-actin.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Generation of a human X-derived minichromosome using telomere-associated chromosome fragmentation.The EMBO journal · 1995Article
- The coding sequences of mouse H2A and H3 histone genes contains a conserved seven nucleotide element that interacts with nuclear factors and is necessary for normal expression.Nucleic acids research · 1995Article
- AP-1, ETS, and transcriptional silencers regulate retinoic acid-dependent induction of keratin 18 in embryonic cells.Molecular and cellular biology · 1994Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have examined the expression of transfected human hypoxanthine phosphoribosyltransferase minigenes (HPRT) in mouse embryonic stem (ES) cells. cDNA constructs of this gene that have been successfully used in somatic cell lines failed to confer hypoxanthine/aminopterin/thymidine (HAT) resistance in ES cells. In contrast, constructs containing introns 1 and 2 from the HPRT gene produced a high frequency of HAT-resistant colonies. This observation allowed us to identify two sequences in these introns that influence expression of the HPRT gene in ES cells. One element, located in intron 2, is required for effective HPRT expression in these cells; the other element, located in intron 1, acts as an enhancer of HPRT expression. Using this information, we have constructed an HPRT minigene that can be used for either positive or negative selection in ES cell experiments. This dual capability allows the design of "in-out" procedures to create subtle changes in target genes by homologous recombination with the aid of this selectable minigene.
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