ArticleMolecular vision2012
A gene expression profile of the developing human retinal pigment epithelium.
Article in Molecular vision, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- 1q gain bypasses the selective barrier against aneuploidy in RPE differentiation via wild-type co-culture rescue.Nature communications · 2025Article
- Influence of Substrate Stiffness on iPSC-Derived Retinal Pigmented Epithelial Cells.Stem cells translational medicine · 2024Article
- Article
- The mouse retinal pigment epithelium mounts an innate immune defense response following retinal detachment.Journal of neuroinflammation · 2024Article
- Elastin turnover in ocular diseases: A special focus on age-related macular degeneration.Experimental eye research · 2022Review
- Soy Protein Nanofiber Scaffolds for Uniform Maturation of Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium.Tissue engineering. Part C, Methods · 2020Article
- Dynamic regulation of histone modifications and long-range chromosomal interactions during postmitotic transcriptional reactivation.Genes & development · 2020Article
- Identification of Key Genes and Pathways Associated with Age-Related Macular Degeneration.Journal of ophthalmology · 2020Article
- Enhancing Understanding of the Visual Cycle by Applying CRISPR/Cas9 Gene Editing in Zebrafish.Frontiers in cell and developmental biology · 2018Review
- An integrative analysis of reprogramming in human isogenic system identified a clone selection criterion.Cell cycle (Georgetown, Tex.) · 2016Article
- Effect of Storage Temperature on Key Functions of Cultured Retinal Pigment Epithelial Cells.Journal of ophthalmology · 2015Article
- Differential Gene Expression in Age-Related Macular Degeneration.Cold Spring Harbor perspectives in medicine · 2014Review
- Human pluripotent stem cell-derived retinal pigmented epithelium in retinal treatment: from bench to bedside.Molecular neurobiology · 2014Review
- Expression profiling of the RPE in zebrafish smarca4 mutant revealed altered signals that potentially affect RPE and retinal differentiation.Molecular vision · 2014Article
- Methods for culturing retinal pigment epithelial cells: a review of current protocols and future recommendations.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe molecular mechanisms associated with human retinal pigment epithelium (RPE) development constitute the basis for cell replacement therapy for the treatment of retinal degenerative diseases. In the current study, gene expression analysis of the human fetal RPE during development was performed and was compared with the human native RPE.
methodsMicrodissection of the human RPE at three time points (13 weeks and 16 weeks of gestation and in mature adult eyes) was performed, and total RNA was isolated. Equal amounts of RNA were pooled from two or three independent donor eyes for each time point in each group. Gene expression was analyzed by hybridization to microarray chips. Validation was accomplished by comparing the microarray expression profiles with quantitative real-time reverse transcriptase-polymerase chain reaction (qRT(2)-PCR) analysis of selected genes and by comparing selected expression profiles with predicted profiles based on previous studies.
resultsOf the 45,033 probe sets on the microarray, 30,736 were detected. A total of 3,498 differentially expressed genes could be clustered into eight patterns of expression that were statistically significant. Analysis of the expression patterns of genes coding for key functions (pigment synthesis, visual cycle, phagocytosis, adherens and tight junctions, and transcellular transport) indicated that the human RPE achieves a high degree of maturity during early pregnancy. Compared with 154 signature genes in the RPE, 148 candidate genes were identified in this study, including 53 downregulated genes and 5 upregulated genes. The qRT(2)-PCR results showed similar expression trends to those obtained by microarray analysis at the three time points.
conclusionsThis study demonstrated gene expression profiles in the human RPE during normal development. These findings indicate that the human RPE has different expression patterns than those of other animals. The results of this study may be helpful in furthering the understanding of the developmental processes occurring in humans and of the differentiation of RPE cells derived from human embryonic stem cells and from human induced pluripotent stem cells.
Indexed as
Identifiers
23487591PMC3594943W2139548676What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.