ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2013
Intracellular magnesium-dependent modulation of gap junction channels formed by neuronal connexin36.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 63 citations in OpenAlex.
- Divalent cation depletion enhances intrinsic neuronal excitability through CaSR-dependent modulation of threshold ion channels.PNAS nexus · 2026Article
- Quantifying electrostatic control of docking and binding energetics in functional Cx36 gap junctions.Communications biology · 2026Article
- Influence of extracellular magnesium ions on the functional state and biophysical and structural properties of connexin36 hemichannels.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Dose- and Time-Dependent Modulation of Cx43 and Cx45 Expression and Gap Junction Conductance by Resveratrol.Antioxidants (Basel, Switzerland) · 2026Article
- The Impact of Chronic Magnesium Deficiency on Excitable Tissues-Translational Aspects.Biological trace element research · 2025Review
- The rectification of heterotypic Cx46/Cx50 gap junction channels depends on intracellular magnesium.Biophysics reports · 2024Article
- Magnesium (MgCurrent pharmaceutical design · 2024Review
- Function and Plasticity of Electrical Synapses in the Mammalian Brain: Role of Non-Junctional Mechanisms.Biology · 2022Review
- The Amino Terminal Domain and Modulation of Connexin36 Gap Junction Channels by Intracellular Magnesium Ions.Frontiers in physiology · 2022Article
- An In Vitro Evaluation of the Biological and Osteogenic Properties of Magnesium-Doped Bioactive Glasses for Application in Bone Tissue Engineering.International journal of molecular sciences · 2021Article
- Convergent NMDA receptor-Pannexin1 signaling pathways regulate the interaction of CaMKII with Connexin-36.Communications biology · 2021Article
- Engineering Smart Hybrid Tissues with Built-In Electronics.iScience · 2020Review
- Magnesium Is a Key Player in Neuronal Maturation and Neuropathology.International journal of molecular sciences · 2019Review
- Design principles of electrical synaptic plasticity.Neuroscience letters · 2019Review
- Midbody: From the Regulator of Cytokinesis to Postmitotic Signaling Organelle.Medicina (Kaunas, Lithuania) · 2018Review
- Gating of Connexin Channels by transjunctional-voltage: Conformations and models of open and closed states.Biochimica et biophysica acta. Biomembranes · 2018Review
- Modulation of Connexin-36 Gap Junction Channels by Intracellular pH and Magnesium Ions.Frontiers in physiology · 2018Article
- Connexins and pannexins: At the junction of neuro-glial homeostasis & disease.Journal of neuroscience research · 2018Review
- Two Forms of Electrical Transmission Between Neurons.Frontiers in molecular neuroscience · 2018Article
- The electrical synapse: Molecular complexities at the gap and beyond.Developmental neurobiology · 2017Review
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
Abstract
Gap junction (GJ) channels composed of Connexin36 (Cx36) are widely expressed in the mammalian CNS and form electrical synapses between neurons. Here we describe a novel modulatory mechanism of Cx36 GJ channels dependent on intracellular free magnesium ([Mg(2+)]i). We examined junctional conductance (gj) and its dependence on transjunctional voltage (Vj) at different [Mg(2+)]i in cultures of HeLa or N2A cells expressing Cx36. We found that Cx36 GJs are partially inhibited at resting [Mg(2+)]i. Thus, gj can be augmented or reduced by lowering or increasing [Mg(2+)]i, respectively. Similar changes in gj and Vj-gating were observed using MgATP or K2ATP in pipette solutions, which increases or decreases [Mg(2+)]i, respectively. Changes in phosphorylation of Cx36 or in intracellular free calcium concentration were not involved in the observed Mg(2+)-dependent modulation of gj. Magnesium ions permeate the channel and transjunctional asymmetry in [Mg(2+)]i resulted in asymmetric Vj-gating. The gj of GJs formed of Cx26, Cx32, Cx43, Cx45, and Cx47 was also reduced by increasing [Mg(2+)]i, but was not increased by lowering [Mg(2+)]i; single-channel conductance did not change. We showed that [Mg(2+)]i affects both open probability and the number of functional channels, likely through binding in the channel lumen. Finally, we showed that Cx36-containing electrical synapses between neurons of the trigeminal mesencephalic nucleus in rat brain slices are similarly affected by changes in [Mg(2+)]i. Thus, this novel modulatory mechanism could underlie changes in neuronal synchronization under conditions in which ATP levels, and consequently [Mg(2+)]i, are modified.
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