ArticleInternational journal of nanomedicine2013
Visualization of internalization of functionalized cobalt ferrite nanoparticles and their intracellular fate.
Article in International journal of nanomedicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Phases of tight junction barrier disruption during transurothelial migration of invasive urothelial cancer cells.Scientific reports · 2025Article
- Importance of the electrophoresis and pulse energy for siRNA-mediated gene silencing by electroporation in differentiated primary human myotubes.Biomedical engineering online · 2024Article
- Synthesis and Cytotoxicity Assessment of Citrate-Coated Calcium and Manganese Ferrite Nanoparticles for Magnetic Hyperthermia.Pharmaceutics · 2022Article
- Cobalt Ferrite Magnetic Nanoparticles for Tracing Mesenchymal Stem Cells in Tissue: A Preliminary Study.International journal of molecular sciences · 2022Article
- The Relevance of Physico-Chemical Properties and Protein Corona for Evaluation of Nanoparticles Immunotoxicity-In Vitro Correlation Analysis on THP-1 Macrophages.International journal of molecular sciences · 2022Article
- Article
- How Cancer Cells Invade Bladder Epithelium and Form Tumors: The Mouse Bladder Tumor Model as a Model of Tumor Recurrence in Patients.International journal of molecular sciences · 2021Article
- Attachment of Cancer Urothelial Cells to the Bladder Epithelium Occurs on Uroplakin-Negative Cells and Is Mediated by Desmosomal and Not by Classical Cadherins.International journal of molecular sciences · 2021Article
- In vivo Cell Tracking Using Non-invasive Imaging of Iron Oxide-Based Particles with Particular Relevance for Stem Cell-Based Treatments of Neurological and Cardiac Disease.Molecular imaging and biology · 2020Review
- Proposing Urothelial and Muscle In Vitro Cell Models as a Novel Approach for Assessment of Long-Term Toxicity of Nanoparticles.International journal of molecular sciences · 2020Article
- Analysis of the Direct and Indirect Effects of Nanoparticle Exposure on Microglial and Neuronal Cells In Vitro.International journal of molecular sciences · 2020Article
- How cancer cells attach to urinary bladder epithelium in vivo: study of the early stages of tumorigenesis in an orthotopic mouse bladder tumor model.Histochemistry and cell biology · 2019Article
- Increased endocytosis of magnetic nanoparticles into cancerous urothelial cells versus normal urothelial cells.Histochemistry and cell biology · 2018Article
- The Effect of Different Types of Nanoparticles on FUS and TDP-43 Solubility and Subcellular Localization.Neurotoxicity research · 2017Article
- Short single-stranded DNA degradation products augment the activation of Toll-like receptor 9.Nature communications · 2017Article
- Dispersion of Nanoparticles in Different Media Importantly Determines the Composition of Their Protein Corona.PloS one · 2017Article
- Cellular uptake, genotoxicity and cytotoxicity of cobalt ferrite magnetic nanoparticles in human breast cells.Toxicology research · 2016Article
- Magnetofection Enhances Adenoviral Vector-based Gene Delivery in Skeletal Muscle Cells.Journal of nanomedicine & nanotechnology · 2016Article
- Cobalt oxide nanoparticles can enter inside the cells by crossing plasma membranes.Scientific reports · 2016Article
- Semi-automated quantification of living cells with internalized nanostructures.Journal of nanobiotechnology · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, nanoparticles (NPs) and related applications have become an intensive area of research, especially in the biotechnological and biomedical fields, with magnetic NPs being one of the promising tools for tumor treatment and as MRI-contrast enhancers. Several internalization and cytotoxicity studies have been performed, but there are still many unanswered questions concerning NP interactions with cells and NP stability. In this study, we prepared functionalized magnetic NPs coated with polyacrylic acid, which were stable in physiological conditions and which were also nontoxic short-term. Using fluorescence, scanning, and transmission electron microscopy, we were able to observe and determine the internalization pathways of polyacrylic acid-coated NPs in Chinese hamster ovary cells. With scanning electron microscopy we captured what might be the first step of NPs internalization - an endocytic vesicle in the process of formation enclosing NPs bound to the membrane. With fluorescence microscopy we observed that NP aggregates were rapidly internalized, in a time-dependent manner, via macropinocytosis and clathrin-mediated endocytosis. Inside the cytoplasm, aggregated NPs were found enclosed in acidified vesicles accumulated in the perinuclear region 1 hour after exposure, where they stayed for up to 24 hours. High intracellular loading of NPs in the Chinese hamster ovary cells was obtained after 24 hours, with no observable toxic effects. Thus polyacrylic acid-coated NPs have potential for use in biotechnological and biomedical applications.
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