ArticleStem cells translational medicine2013
Recellularization of acellular human small intestine using bone marrow stem cells.
Article in Stem cells translational medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Tissue-Engineered Grafts from Human Decellularized Extracellular Matrices: A Systematic Review and Future Perspectives.International journal of molecular sciences · 2018Pooled it
- Why retracted articles can have an influential afterlife-and what to do about it.Stem cells translational medicine · 2026Article
- Next-Generation Therapies for Gastrointestinal Repair: Converging Traditional Medicine and Tissue Engineering.JGH open : an open access journal of gastroenterology and hepatology · 2025Review
- Towards Safety and Regulation Criteria for Clinical Applications of Decellularized Organ-Derived Matrices.Bioengineering (Basel, Switzerland) · 2025Review
- Decellularized adipose matrix provides an inductive microenvironment for stem cells in tissue regeneration.World journal of stem cells · 2020Review
- Extracellular Matrix-Based Biomaterials and Their Influence Upon Cell Behavior.Annals of biomedical engineering · 2020Article
- Comparison of polyglycolic acid, polycaprolactone, and collagen as scaffolds for the production of tissue engineered intestine.Journal of biomedical materials research. Part B, Applied biomaterials · 2019Article
- Advances and Current Challenges in IntestinalFrontiers in bioengineering and biotechnology · 2019Review
- Gut-microbiota-on-a-chip: an enabling field for physiological research.Microphysiological systems · 2018Article
- Induction of lung-like cells from mouse embryonic stem cells by decellularized lung matrix.Biochemistry and biophysics reports · 2018Article
- Decellularised skeletal muscles allow functional muscle regeneration by promoting host cell migration.Scientific reports · 2018Article
- Stem cell therapy in necrotizing enterocolitis: Current state and future directions.Seminars in pediatric surgery · 2018Review
- Extracellular Matrix Bioscaffolds for Building Gastrointestinal Tissue.Cellular and molecular gastroenterology and hepatology · 2018Review
- Composite Scaffolds Based on Intestinal Extracellular Matrices and Oxidized Polyvinyl Alcohol: A Preliminary Study for a New Regenerative Approach in Short Bowel Syndrome.BioMed research international · 2018Article
- Significantly Accelerated Wound Healing of Full-Thickness Skin Using a Novel Composite Gel of Porcine Acellular Dermal Matrix and Human Peripheral Blood Cells.Cell transplantation · 2017Article
- Current achievements and future perspectives in whole-organ bioengineering.Stem cell research & therapy · 2015Review
- Bioengineering for Organ Transplantation: Progress and Challenges.Bioengineered · 2015Review
- Decellularized scaffolds as a platform for bioengineered organs.Current opinion in organ transplantation · 2014Review
Corrections and comments
- Retraction · 2019-02-01Concerns/Issues about Image · Unreliable Image · · see also https://pubpeer.com/publications/D4D480B3FC85FF68A139A4004A2C01?utm_source=Chrome&utm_medium=BrowserExtension&utm_campaign=Chrome
- Retracted
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We aimed to produce an acellular human tissue scaffold with a view to test the possibility of recellularization with bone marrow stem cells to produce a tissue-engineered small intestine (TESI). Human small-bowel specimens (n = 5) were obtained from cadaveric organ donors and treated sequentially with 6% dimethyl sulfoxide in hypotonic buffer, 1% Triton X-100, and DNase. Each small intestine (SI) piece (6 cm) was recellularized with EPCAM+ and CD133+ allogeneic bone marrow stem cells. Histological and molecular analysis demonstrated that after decellularization, all cellular components and nuclear material were removed. Our analysis also showed that the decellularized human SI tissue retained its histoarchitecture with intact villi and major structural proteins. Protein films of common extracellular matrix constituents (collagen I, laminin, and fibronectin) were found in abundance. Furthermore, several residual angiogenic factors were found in the decellularized SI. Following recellularization, we found viable mucin-positive goblet cells, CK18+ epithelial cells in villi adjacent to a muscularis mucosa with α-actin+ smooth muscle cells, and a high repopulation of blood vessels with CD31+ endothelial cells. Our results show that in the future, such a TESI would be ideal for clinical purposes, because it can be derived from the recipient's own immunocompatible bone marrow cells, thus avoiding the use of immunosuppression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.