ArticleThe Journal of biological chemistry2013
Light- and metabolism-related regulation of the chloroplast ATP synthase has distinct mechanisms and functions.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 56 citations in OpenAlex.
- Electron transport reactions of cyanobacterial photosynthesis and state-of-the-art in vivo measurement techniques.Plant physiology · 2026Review
- The role of photorespiration in preventing feedback regulation via ATP synthase in Nicotiana tabacum.Plant, cell & environment · 2024Article
- Dependence of state transitions on illumination time in arabidopsis and barley plants.Protoplasma · 2024Article
- High cyclic electron transfer via the PGR5 pathway in the absence of photosynthetic control.Plant physiology · 2023Article
- Characterization of mutants deficient in N-terminal phosphorylation of the chloroplast ATP synthase subunit β.Plant physiology · 2023Article
- Two specific domains of the γ subunit of chloroplast FProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions.International journal of molecular sciences · 2023Article
- Dissipation of the proton electrochemical gradient in chloroplasts promotes the oxidation of ATP synthase by thioredoxin-like proteins.The Journal of biological chemistry · 2022Article
- Verification of the Relationship between Redox Regulation of Thioredoxin Target Proteins and Their Proximity to Thylakoid Membranes.Antioxidants (Basel, Switzerland) · 2022Article
- Inactivation of mitochondrial complex I stimulates chloroplast ATPase in Physcomitrium patens.Plant physiology · 2021Article
- Collaboration between NDH and KEA3 Allows Maximally Efficient Photosynthesis after a Long Dark Adaptation.Plant physiology · 2020Article
- Modification of Activity of the Thylakoid HPlant physiology · 2019Article
- Comparative Proteomic Analysis Reveals Elevated Capacity for Photosynthesis in Polyphenol Oxidase Expression-SilencedInternational journal of molecular sciences · 2018Article
- Proteome Profiling of Paulownia Seedlings Infected with Phytoplasma.Frontiers in plant science · 2017Article
- Chloroplast ATP Synthase Modulation of the Thylakoid Proton Motive Force: Implications for Photosystem I and Photosystem II Photoprotection.Frontiers in plant science · 2017Article
- The Role of Light-Dark Regulation of the Chloroplast ATP Synthase.Frontiers in plant science · 2017Article
- MultispeQ Beta: a tool for large-scale plant phenotyping connected to the open PhotosynQ network.Royal Society open science · 2016Article
- Article
- Sub-Cellular Localization and Complex Formation by Aminoacyl-tRNA Synthetases in Cyanobacteria: Evidence for Interaction of Membrane-Anchored ValRS with ATP Synthase.Frontiers in microbiology · 2016Article
- Purification and biochemical characterization of the ATP synthase from Heliobacterium modesticaldum.Protein expression and purification · 2015Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chloroplast CF0-CF1-ATP synthase (ATP synthase) is activated in the light and inactivated in the dark by thioredoxin-mediated redox modulation of a disulfide bridge on its γ subunit. The activity of the ATP synthase is also fine-tuned during steady-state photosynthesis in response to metabolic changes, e.g. altering CO2 levels to adjust the thylakoid proton gradient and thus the regulation of light harvesting and electron transfer. The mechanism of this fine-tuning is unknown. We test here the possibility that it also involves redox modulation. We found that modifying the Arabidopsis thaliana γ subunit by mutating three highly conserved acidic amino acids, D211V, E212L, and E226L, resulted in a mutant, termed mothra, in which ATP synthase which lacked light-dark regulation had relatively small effects on maximal activity in vivo. In situ equilibrium redox titrations and thiol redox-sensitive labeling studies showed that the γ subunit disulfide/sulfhydryl couple in the modified ATP synthase has a more reducing redox potential and thus remains predominantly oxidized under physiological conditions, implying that the highly conserved acidic residues in the γ subunit influence thiol redox potential. In contrast to its altered light-dark regulation, mothra retained wild-type fine-tuning of ATP synthase activity in response to changes in ambient CO2 concentrations, indicating that the light-dark- and metabolic-related regulation occur through different mechanisms, possibly via small molecule allosteric effectors or covalent modification.
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