ArticleThe Journal of biological chemistry2013
Electrostatics control actin filament nucleation and elongation kinetics.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- On the Assembly of Actin Polymerization-Powered Motors.Macromolecular rapid communications · 2026Article
- Molecular Basis for Actin Polymerization Kinetics Modulated by Solution Crowding.Biomolecules · 2023Article
- Depletion plays a pivotal role in self-incompatibility, revealing a link between cellular energy status, cytosolic acidification and actin remodelling in pollen tubes.The New phytologist · 2022Article
- In vitro Preparation of Homogenous Actin Filaments for Dynamic and Electrophoretic Light Scattering Measurements.Bio-protocol · 2022Article
- Hydrodynamic and Polyelectrolyte Properties of Actin Filaments: Theory and Experiments.Polymers · 2022Article
- Article
- Analysis tools for single-monomer measurements of self-assembly processes.Scientific reports · 2022Article
- Structure and function of an atypical homodimeric actin capping protein from the malaria parasite.Cellular and molecular life sciences : CMLS · 2022Article
- pH-Triggered Assembly of Endomembrane Multicompartments in Synthetic Cells.ACS synthetic biology · 2022Article
- Zero-mode waveguides visualize the first steps during gelsolin-mediated actin filament formation.Biophysical journal · 2022Article
- Celebrating 20 years of live single-actin-filament studies with five golden rules.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Self-assembly of protein superstructures by physical interactions under cytoplasm-like conditions.Biophysical journal · 2021Article
- Changes in microtubule stability in zebrafish (Heliyon · 2021Article
- Alkalinization of the Synaptic Cleft during Excitatory Neurotransmission.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Prebiotic competition and evolution in self-replicating polynucleotides can explain the properties of DNA/RNA in modern living systems.BMC evolutionary biology · 2020Article
- Brownian Dynamics Simulations of Biological Molecules.Trends in chemistry · 2019Article
- Calibrating Evanescent-Wave Penetration Depths for Biological TIRF Microscopy.Biophysical journal · 2019Review
- Actin stabilizing compounds show specific biological effects due to their binding mode.Scientific reports · 2019Article
- Factors Affecting Ultrastructural Quality in the Prefrontal Cortex of the Postmortem Human Brain.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2019Article
- Quantitative Variations with pH of Actin Depolymerizing Factor/Cofilin's Multiple Actions on Actin Filaments.Biochemistry · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The actin cytoskeleton is a central mediator of cellular morphogenesis, and rapid actin reorganization drives essential processes such as cell migration and cell division. Whereas several actin-binding proteins are known to be regulated by changes in intracellular pH, detailed information regarding the effect of pH on the actin dynamics itself is still lacking. Here, we combine bulk assays, total internal reflection fluorescence microscopy, fluorescence fluctuation spectroscopy techniques, and theory to comprehensively characterize the effect of pH on actin polymerization. We show that both nucleation and elongation are strongly enhanced at acidic pH, with a maximum close to the pI of actin. Monomer association rates are similarly affected by pH at both ends, although dissociation rates are differentially affected. This indicates that electrostatics control the diffusional encounter but not the dissociation rate, which is critical for the establishment of actin filament asymmetry. A generic model of protein-protein interaction, including electrostatics, explains the observed pH sensitivity as a consequence of charge repulsion. The observed pH effect on actin in vitro agrees with measurements of Listeria propulsion in pH-controlled cells. pH regulation should therefore be considered as a modulator of actin dynamics in a cellular environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.