ArticleMolecular biology of the cell2013
Measurements of forces produced by the mitotic spindle using optical tweezers.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Analyses of bent spindles reveal the mechanics of anaphase B in fission yeast.Molecular biology of the cell · 2026Article
- Analyses of bent spindles reveal the mechanics of anaphase B in fission yeast.bioRxiv : the preprint server for biology · 2025Article
- Shining Light in Mechanobiology: Optical Tweezers, Scissors, and Beyond.ACS photonics · 2024Review
- PICH acts as a force-dependent nucleosome remodeler.Nature communications · 2022Article
- Review
- Blocking Protein Phosphatase 1 [PP1] Prevents Loss of Tether Elasticity in Anaphase Crane-Fly Spermatocytes.Frontiers in molecular biosciences · 2021Article
- Mechanics of Multicentrosomal Clustering in Bipolar Mitotic Spindles.Biophysical journal · 2020Article
- The Mechanics of Mitotic Cell Rounding.Frontiers in cell and developmental biology · 2020Review
- Laser Scissors and Tweezers to Study Chromosomes: A Review.Frontiers in bioengineering and biotechnology · 2020Review
- Technique of laser chromosome welding for chromosome repair and artificial chromosome creation.Biomedical optics express · 2018Article
- Mitotic tethers connect sister chromosomes and transmit "cross-polar" force during anaphase A of mitosis in PtK2 cells.Biomedical optics express · 2017Article
- Review
- Chromosome biorientation produces hundreds of piconewtons at a metazoan kinetochore.Nature communications · 2016Article
- The spindle checkpoint and chromosome segregation in meiosis.The FEBS journal · 2015Review
- From equator to pole: splitting chromosomes in mitosis and meiosis.Genes & development · 2015Review
- Force is a signal that cells cannot ignore.Molecular biology of the cell · 2014Review
- Determination of motility forces on isolated chromosomes with laser tweezers.Scientific reports · 2014Article
- Direct kinetochore-spindle pole connections are not required for chromosome segregation.The Journal of cell biology · 2014Article
- DNA dynamics and single-molecule biology.Chemical reviews · 2014Review
- Linked in: formation and regulation of microtubule attachments during chromosome segregation.Current opinion in cell biology · 2014Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used a trapping laser to stop chromosome movements in Mesostoma and crane-fly spermatocytes and inward movements of spindle poles after laser cuts across Potorous tridactylus (rat kangaroo) kidney (PtK2) cell half-spindles. Mesostoma spermatocyte kinetochores execute oscillatory movements to and away from the spindle pole for 1-2 h, so we could trap kinetochores multiple times in the same spermatocyte. The trap was focused to a single point using a 63× oil immersion objective. Trap powers of 15-23 mW caused kinetochore oscillations to stop or decrease. Kinetochore oscillations resumed when the trap was released. In crane-fly spermatocytes trap powers of 56-85 mW stopped or slowed poleward chromosome movement. In PtK2 cells 8-mW trap power stopped the spindle pole from moving toward the equator. Forces in the traps were calculated using the equation F = Q'P/c, where P is the laser power and c is the speed of light. Use of appropriate Q' coefficients gave the forces for stopping pole movements as 0.3-2.3 pN and for stopping chromosome movements in Mesostoma spermatocytes and crane-fly spermatocytes as 2-3 and 6-10 pN, respectively. These forces are close to theoretical calculations of forces causing chromosome movements but 100 times lower than the 700 pN measured previously in grasshopper spermatocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.