ArticleBioNanoScience2012
Microwell regulation of pluripotent stem cell self-renewal and differentiation.
Article in BioNanoScience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Geometrical constraints dictate assembly and phenotype of human iPSC-derived motoneuronal spheroids.Stem cell research & therapy · 2025Article
- Semi-automated optimized method to isolate CRISPR/Cas9 edited human pluripotent stem cell clones.Stem cell research & therapy · 2023Article
- Analysis of the effects of bench-scale cell culture platforms and inoculum cell concentrations on PSC aggregate formation and culture.Frontiers in bioengineering and biotechnology · 2023Article
- Fabrication approaches for high-throughput and biomimetic disease modeling.Acta biomaterialia · 2021Review
- Microtissue Geometry and Cell-Generated Forces Drive Patterning of Liver Progenitor Cell Differentiation in 3D.Advanced healthcare materials · 2021Article
- Size-Optimized Microspace Culture Facilitates Differentiation of Mouse Induced Pluripotent Stem Cells into Osteoid-Rich Bone Constructs.Stem cells international · 2020Article
- Emerging Methods for Enhancing Pluripotent Stem Cell Expansion.Frontiers in cell and developmental biology · 2020Review
- Evaluation of the osteogenic differentiation of gingiva-derived stem cells grown on culture plates or in stem cell spheroids: Comparison of two- and three-dimensional cultures.Experimental and therapeutic medicine · 2017Article
- Evaluation of the maintenance of stemness, viability, and differentiation potential of gingiva-derived stem-cell spheroids.Experimental and therapeutic medicine · 2017Article
- Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests.Biomedical reports · 2016Article
- Technology advancement for integrative stem cell analyses.Tissue engineering. Part B, Reviews · 2014Review
- Effects of 3D microwell culture on initial fate specification in human embryonic stem cells.AIChE journal. American Institute of Chemical Engineers · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The fates of pluripotent stem cells (PSCs), including survival, self-renewal, and differentiation, are regulated by chemical and mechanical cues presented in the three-dimensional (3D) microenvironment. Most PSC studies have been performed on two-dimensional substrates. However, 3D culture systems have demonstrated the importance of intercellular interactions in regulating PSC self-renewal and differentiation. Microwell culture systems have been developed to generate homogenous PSC colonies of defined sizes and shapes and to study how colony morphology affects cell fate. Using microwells, researchers have demonstrated that PSCs remain in a self-renewing undifferentiated state as a result of autocrine and paracrine signaling. Other studies have shown that microwell regulation of embryoid body size affects lineage commitment during differentiation via cell-cell contact and expression of soluble signals. In this review, we discuss recent advances in the design and utilization of 3D microwell platforms for studying intercellular regulation of PSC cell fate decisions and the underlying molecular mechanisms.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.