ArticleYeast (Chichester, England)2013
Enhancement of plasmid DNA transformation efficiencies in early stationary-phase yeast cell cultures.
Article in Yeast (Chichester, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Protocol for mapping gene essentiality changes in yeast using inducible gene deletion mutants.STAR protocols · 2026Article
- Article
- Cytoplasmic circular dsDNA is a key constituent of stress granules.bioRxiv : the preprint server for biology · 2026Article
- Influence of Ploidy and Genetic Background on Stress Tolerance of Intraspecific Yeast Hybrids.Microbial biotechnology · 2026Article
- A neuregulin-like ligand and EGF receptor underpinFrontiers in cellular and infection microbiology · 2026Article
- SCEP3 initiates synapsis and implements crossover interference in Arabidopsis.Nature plants · 2025Article
- A step-by-step approach to establishing an efficient genetic transformation protocol for Chlorella vulgaris using electroporation.Molecular biology reports · 2025Article
- Closed and open structures of the eukaryotic magnesium channel Mrs2 reveal the auto-ligand-gating regulation mechanism.Nature structural & molecular biology · 2025Article
- The structure and function of P5A-ATPases.Nature communications · 2024Article
- Mapping the structural landscape of the yeast Ty3 retrotransposon RNA genome.Nucleic acids research · 2024Article
- Cross-feeding promotes heterogeneity within yeast cell populations.Nature communications · 2024Article
- A fine-tuned yeast surface-display/secretion platform enables the rapid discovery of neutralizing antibodies against Clostridioides difficile toxins.Microbial cell factories · 2023Article
- Deficiency in homologous recombination is associated with changes in cell cycling and morphology in Saccharomyces cerevisiae.Experimental cell research · 2023Article
- Targeting Echinococcus multilocularis PIM kinase for improving anti-parasitic chemotherapy.PLoS neglected tropical diseases · 2022Article
- High-Efficiency Plasmid DNA Transformation in Yeast.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Suppression of telomere capping defects of Saccharomyces cerevisiae yku70 and yku80 mutants by telomerase.G3 (Bethesda, Md.) · 2021Article
- Efficient breeding of industrial brewing yeast strains using CRISPR/Cas9-aided mating-type switching.Applied microbiology and biotechnology · 2021Article
- Structure and transport mechanism of P5B-ATPases.Nature communications · 2021Article
- Towards deorphanizing G protein-coupled receptors ofParasitology · 2020Article
- 2'-O-ribose methylation of transfer RNA promotes recovery from oxidative stress in Saccharomyces cerevisiae.PloS one · 2020Article
Corrections and comments
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4 authors.
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Abstract
Chemical-based methods have been developed for transformation of DNA into log-phase cells of the budding yeast Saccharomyces cerevisiae with high efficiency. Transformation of early stationary-phase cells, e.g. cells grown in overnight liquid cultures or as colonies on plates, is less efficient than log-phase cells but is simpler and more adaptable to high-throughput projects. In this study we have tested different approaches for transformation of early stationary-phase cell cultures and identified a method utilizing polyethylene glycol (PEG), lithium acetate and dimethyl sulphoxide (DMSO) as the most efficient. Plasmid DNA transformations using this method could be improved modestly by allowing cells to recover from the chemical treatment in rich broth before plating to selective media. Strong increases in transformation efficiencies were observed when cells were treated briefly with dithiothreitol (DTT). Tests using several different yeast strain backgrounds indicated that DTT treatment could enhance transformation efficiencies by up to 40-fold. Evaluation of multiple parameters affecting the efficiency of the method led to development of an optimized protocol achieving > 50 000 transformants/µg DNA in most backgrounds tested.
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