ArticleMolecular and cellular biochemistry2013
Folate metabolite profiling of different cell types and embryos suggests variation in folate one-carbon metabolism, including developmental changes in human embryonic brain.
Article in Molecular and cellular biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- A Nonketotic Hyperglycinemia Mouse Shows Wide-Ranging Biochemical Consequences of Elevated Glycine, Reduced Folate One-Carbon Charging, and Serine Deficiency.Journal of inherited metabolic disease · 2026Article
- Mitochondria protect against an intracellular pathogen by restricting access to folate.Science (New York, N.Y.) · 2025Article
- Comprehensive profiling of folates across polyglutamylation and one-carbon states.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- Challenges of folate species analysis in food and biological matrices by liquid chromatography-tandem mass spectrometery.Bioanalysis · 2025Review
- DHFR2 RNA directly regulates dihydrofolate reductase and its expression level impacts folate one carbon metabolism.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- One-carbon metabolism and microbial pathogenicity.Molecular oral microbiology · 2024Review
- Simultaneous Determination of One-Carbon Folate Metabolites and One-Carbon-Related Amino Acids in Biological Samples Using a UHPLC-MS/MS Method.International journal of molecular sciences · 2024Article
- An investigation of the effect of folic acid and its delivery routes on broiler chickens' hatch and growth performance, blood biochemistry, anti-oxidant status, and intestinal morphology.Journal of animal science · 2023Article
- Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.Cellular and molecular life sciences : CMLS · 2022Article
- Comprehensive Vitamer Profiling of Folate Mono- and Polyglutamates in Baker's Yeast (Metabolites · 2020Article
- Mechanical properties measured by atomic force microscopy define health biomarkers in ageing C. elegans.Nature communications · 2020Article
- A new mechanism for cannabidiol in regulating the one-carbon cycle and methionine levels in Dictyostelium and in mammalian epilepsy models.British journal of pharmacology · 2020Article
- Cellular mechanisms underlyingDisease models & mechanisms · 2019Article
- Glycine decarboxylase deficiency-induced motor dysfunction in zebrafish is rescued by counterbalancing glycine synaptic level.JCI insight · 2018Article
- Simultaneous quantitation of folates, flavins and BJournal of pharmaceutical and biomedical analysis · 2018Article
- Partitioning of One-Carbon Units in Folate and Methionine Metabolism Is Essential for Neural Tube Closure.Cell reports · 2017Article
- Finding the genetic mechanisms of folate deficiency and neural tube defects-Leaving no stone unturned.American journal of medical genetics. Part A · 2017Review
- One-Carbon Metabolism in Health and Disease.Cell metabolism · 2017Review
- Article
- Effect of in ovo folic acid injection on hepatic IGF2 expression and embryo growth of broilers.Journal of animal science and biotechnology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Folates act as co-factors for transfer of one-carbon units for nucleotide production, methylation and other biosynthetic reactions. Comprehensive profiling of multiple folates can be achieved using liquid chromatography tandem mass spectrometry, enabling determination of their relative abundance that may provide an indication of metabolic differences between cell types. For example, cell lines exposed to methotrexate showed a dose-dependent elevation of dihydrofolate, consistent with inhibition of dihydrofolate reductase. We analysed the folate profile of E. coli sub-types as well as cell lines and embryonic tissue from both human and mouse. The folate profile of bacteria differed markedly from those of all the mammalian samples, most notably in the greater abundance of formyl tetrahydrofolate. The overall profiles of mouse and human fibroblasts and mid-gestation mouse embryos were broadly similar, with specific differences. The major folate species in these cell types was 5-methyl tetrahydrofolate, in contrast to lymphoblastoid cell lines in which the predominant form was tetrahydrofolate. Analysis of embryonic human brain revealed a shift in folate profile with increasing developmental stage, with a decline in relative abundance of dihydrofolate and increase in 5-methyl tetrahydrofolate. These cell type-specific and developmental changes in folate profile may indicate differential requirements for the various outputs of folate metabolism.
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Registered trials
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