ArticleTransgenic research2013
Mechanism of random integration of foreign DNA in transgenic mice.
Article in Transgenic research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- The precision strategy of human genome correction via a set of circular donor DNA and its cleaver.Frontiers in genome editing · 2026Article
- Advanced human FcRn knock-in mice for pharmacokinetic profiling of therapeutic antibodies.Scientific reports · 2025Article
- Transgene Mapping in Animals: What to Choose?International journal of molecular sciences · 2025Review
- Current status and future of gene engineering in livestock.BMB reports · 2024Review
- CRISPR-KRISPR: a method to identify on-target and random insertion of donor DNAs and their characterization in knock-in mice.Genome biology · 2022Article
- Production of CFTR Mutant Gene Model by Homologous Recombination System.Cell journal · 2022Article
- Considerations for homology-based DNA repair in mosquitoes: Impact of sequence heterology and donor template source.PLoS genetics · 2022Article
- Programmable RNA-Guided Large DNA Transgenesis by CRISPR/Cas9 and Site-Specific Integrase Bxb1.Frontiers in bioengineering and biotechnology · 2022Review
- Article
- Review
- When the genome bluffs: a tandem duplication event during generation of a novel Agmo knockout mouse model fools routine genotyping.Cell & bioscience · 2021Article
- The Progress of CRISPR/Cas9-Mediated Gene Editing in Generating Mouse/Zebrafish Models of Human Skeletal Diseases.Computational and structural biotechnology journal · 2019Review
- Loss of smooth muscle myosin heavy chain results in the bladder and stomach developing lesion during foetal development in mice.Journal of genetics · 2018Article
- Efficient transgenesis and annotated genome sequence of the regenerative flatworm model Macrostomum lignano.Nature communications · 2017Article
- Creating conditional dual fluorescence labeled transgenic animals for studying function of small noncoding RNAs.Connective tissue research · 2017Article
- The integration site of theWellcome open research · 2017Article
- The distribution of cotransformed transgenes in particle bombardment-mediated transformed wheat.Transgenic research · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Little is known about how foreign DNA is randomly integrated into chromosomes in transgenic animals. In the current study, the insertion sites of 36 transgenic mice were mapped by thermal asymmetric interlaced PCR, and 38 junction sequences were obtained from 30 samples. Analysis of the 38 sequences revealed that 44.7 % of integration events occurred within host gene regions, including 13.2 % (5/38) in exonic regions and 31.6 % (12/38) in intronic regions. The results also revealed that all non-end side integrations of foreign DNA were mediated by short sequence homologies (microhomologies) and that the end side integrations occurred in the presence or absence of microhomologies. In addition, microhomology-mediated mechanisms were also confirmed in four transgenic Arabidopsis thaliana lines. The results indicate that foreign DNA is easily integrated into host gene regions. These results also suggest that the integration of both ends of foreign DNA follows the above-mentioned mechanism in many transgenic/transformed organisms.
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