ArticleDevelopment (Cambridge, England)2013
Lethal giant larvae 2 regulates development of the ciliated organ Kupffer's vesicle.
Article in Development (Cambridge, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Checkpoint kinase 2 coordinates autophagy activation and Aurora kinase A degradation to regulate primary cilia for cell invasion.Cell communication and signaling : CCS · 2026Article
- Calcium signaling mediates proliferation of the precursor cells that give rise to the ciliated left-right organizer in the zebrafish embryo.Frontiers in molecular biosciences · 2023Article
- Understanding laterality disorders and the left-right organizer: Insights from zebrafish.Frontiers in cell and developmental biology · 2022Review
- The Drosophila actin nucleator DAAM is essential for left-right asymmetry.PLoS genetics · 2020Article
- Cytokinetic bridge triggers de novo lumen formation in vivo.Nature communications · 2020Article
- Akap12beta supports asymmetric heart development via modulating the Kupffer's vesicle formation in zebrafish.BMB reports · 2019Article
- Article
- Aplnra/b Sequentially Regulate Organ Left-Right Patterning via Distinct Mechanisms.International journal of biological sciences · 2019Article
- Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer's vesicle.eLife · 2018Article
- Mutations in zebrafish pitx2 model congenital malformations in Axenfeld-Rieger syndrome but do not disrupt left-right placement of visceral organs.Developmental biology · 2016Article
- Article
- Kupffer's vesicle size threshold for robust left-right patterning of the zebrafish embryo.Developmental dynamics : an official publication of the American Association of Anatomists · 2016Article
- The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish.Developmental biology · 2015Article
- Cell collectivity regulation within migrating cell cluster during Kupffer's vesicle formation in zebrafish.Frontiers in cell and developmental biology · 2015Review
- Functional characterization of Prickle2 and BBS7 identify overlapping phenotypes yet distinct mechanisms.Developmental biology · 2014Article
- Orphan G-protein coupled receptor 22 (Gpr22) regulates cilia length and structure in the zebrafish Kupffer's vesicle.PloS one · 2014Article
- Ethanol exposure disrupts extraembryonic microtubule cytoskeleton and embryonic blastomere cell adhesion, producing epiboly and gastrulation defects.Biology open · 2013Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
Motile cilia perform crucial functions during embryonic development and throughout adult life. Development of organs containing motile cilia involves regulation of cilia formation (ciliogenesis) and formation of a luminal space (lumenogenesis) in which cilia generate fluid flows. Control of ciliogenesis and lumenogenesis is not yet fully understood, and it remains unclear whether these processes are coupled. In the zebrafish embryo, lethal giant larvae 2 (lgl2) is expressed prominently in ciliated organs. Lgl proteins are involved in establishing cell polarity and have been implicated in vesicle trafficking. Here, we identified a role for Lgl2 in development of ciliated epithelia in Kupffer's vesicle, which directs left-right asymmetry of the embryo; the otic vesicles, which give rise to the inner ear; and the pronephric ducts of the kidney. Using Kupffer's vesicle as a model ciliated organ, we found that depletion of Lgl2 disrupted lumen formation and reduced cilia number and length. Immunofluorescence and time-lapse imaging of Kupffer's vesicle morphogenesis in Lgl2-deficient embryos suggested cell adhesion defects and revealed loss of the adherens junction component E-cadherin at lateral membranes. Genetic interaction experiments indicate that Lgl2 interacts with Rab11a to regulate E-cadherin and mediate lumen formation that is uncoupled from cilia formation. These results uncover new roles and interactions for Lgl2 that are crucial for both lumenogenesis and ciliogenesis and indicate that these processes are genetically separable in zebrafish.
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