ArticleAmerican journal of physiology. Endocrinology and metabolism2013
Loss of neuronatin promotes "browning" of primary mouse adipocytes while reducing Glut1-mediated glucose disposal.
Article in American journal of physiology. Endocrinology and metabolism, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Exploring the role of epicardial adipose-tissue-derived extracellular vesicles in cardiovascular diseases.iScience · 2024Review
- Circulating Neuronatin Levels Are Positively Associated with BMI and Body Fat Mass but Not with Psychological Parameters.Nutrients · 2023Article
- Mother's warmth from maternal genes: genomic imprinting of brown adipose tissue.Evolution, medicine, and public health · 2023Article
- Depot-specific adaption of adipose tissue for different exercise approaches in high-fat diet/streptozocin-induced diabetic mice.Frontiers in physiology · 2023Article
- Independent phenotypic plasticity axes define distinct obesity sub-types.Nature metabolism · 2022Article
- Identification of Smad3-related transcriptomes in type-2 diabetic nephropathy by whole transcriptome RNA sequencing.Journal of cellular and molecular medicine · 2021Article
- Neuronatin regulates whole-body metabolism: is thermogenesis involved?FASEB bioAdvances · 2020Article
- A FACS-based approach to obtain viable eosinophils from human adipose tissue.Scientific reports · 2020Article
- Characteristic Localization of Neuronatin in Rat Testis, Hair Follicle, Tongue, and Pancreas.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2019Article
- Polyphenism - A Window Into Gene-Environment Interactions and Phenotypic Plasticity.Frontiers in genetics · 2019Review
- Effect of Long-Term Exercise Training on lncRNAs Expression in the Vascular Injury of Insulin Resistance.Journal of cardiovascular translational research · 2018Article
- Neuronatin deletion causes postnatal growth restriction and adult obesity in 129S2/Sv mice.Molecular metabolism · 2018Article
- Cartilage rings contribute to the proper embryonic tracheal epithelial differentiation, metabolism, and expression of inflammatory genes.American journal of physiology. Lung cellular and molecular physiology · 2017Article
- Metabolic heterogeneity of activated beige/brite adipocytes in inguinal adipose tissue.Scientific reports · 2017Article
- Biomarkers of browning of white adipose tissue and their regulation during exercise- and diet-induced weight loss.The American journal of clinical nutrition · 2016Article
- Effects of hibernation on bone marrow transcriptome in thirteen-lined ground squirrels.Physiological genomics · 2016Article
- iGEMS: an integrated model for identification of alternative exon usage events.Nucleic acids research · 2016Article
- Apoptotic transition of senescent cells accompanied with mitochondrial hyper-function.Oncotarget · 2016Article
- White-to-brite conversion in human adipocytes promotes metabolic reprogramming towards fatty acid anabolic and catabolic pathways.Molecular metabolism · 2016Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Failure of white adipose tissue to appropriately store excess metabolic substrate seems to underpin obesity-associated type 2 diabetes. Encouraging "browning" of white adipose has been suggested as a therapeutic strategy to help dispose of excess stored lipid and ameliorate the resulting insulin resistance. Genetic variation at the DNA locus encoding the novel proteolipid neuronatin has been associated with obesity, and we recently observed that neuronatin expression is reduced in subcutaneous adipose tissue from obese humans. Thus, to explore the function of neuronatin further, we used RNAi to silence its expression in murine primary adipocyte cultures and examined the effects on adipocyte phenotype. We found that primary adipocytes express only the longer isoform of neuronatin. Loss of neuronatin led to increased mitochondrial biogenesis, indicated by greater intensity of MitoTracker Green staining. This was accompanied by increased expression of UCP1 and the key genes in mitochondrial oxidative phosphorylation, PGC-1α, Cox8b, and Cox4 in primary subcutaneous white adipocytes, indicative of a "browning" effect. In addition, phosphorylation of AMPK and ACC was increased, suggestive of increased fatty acid utilization. Similar, but less pronounced, effects of neuronatin silencing were also noted in primary brown adipocytes. In contrast, loss of neuronatin caused a reduction in both basal and insulin-stimulated glucose uptake and glycogen synthesis, likely mediated by a reduction in Glut1 protein upon silencing of neuronatin. In contrast, loss of neuronatin had no effect on insulin signaling. In conclusion, neuronatin appears to be a novel regulator of browning and metabolic substrate disposal in white adipocytes.
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