ArticleMolecular microbiology2013
DNA methylation by CcrM activates the transcription of two genes required for the division of Caulobacter crescentus.
Article in Molecular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 70 citations in OpenAlex.
- Genome replication and cell division control by the methylation-sensitive GcrA regulator in Agrobacterium tumefaciens.Nucleic acids research · 2026Article
- Type I restriction-modification systems regulate gene expression through DNA topology.Nucleic acids research · 2026Article
- Comparative genomics and methylome profiling ofMicrobial genomics · 2026Article
- Cytosine methylation contributes to the fitness ofiScience · 2026Article
- Long-read methylome analysis of Oleidesulfovibrio alaskensis G20 biofilm under copper stress.Scientific reports · 2025Article
- Methylome-driven regulation of miRNA expression and its relationship to cardiac dysfunction in idiopathic dilated cardiomyopathy.Clinical epigenetics · 2025Article
- Article
- DNA methylation by CcrM contributes to genome maintenance in the Agrobacterium tumefaciens plant pathogen.Nucleic acids research · 2024Article
- Review
- AamA-mediated epigenetic control of genome-wide gene expression and phenotypic traits inMicrobial genomics · 2023Article
- Antimicrobial resistance and mechanisms of epigenetic regulation.Frontiers in cellular and infection microbiology · 2023Review
- Bacterial DNA methyltransferase: A key to the epigenetic world with lessons learned from proteobacteria.Frontiers in microbiology · 2023Review
- DNA Methylation inmSystems · 2022Article
- The noncoding RNA CcnA modulates the master cell cycle regulators CtrA and GcrA in Caulobacter crescentus.PLoS biology · 2022Article
- Advanced biotechnology using methyltransferase and its applications in bacteria: a mini review.Biotechnology letters · 2022Review
- DNA Methylation in Prokaryotes.Advances in experimental medicine and biology · 2022Article
- Computational modeling of unphosphorylated CtrA:iScience · 2021Article
- Regulator-dependent temporal dynamics of a restriction-modification system's gene expression upon entering new host cells: single-cell and population studies.Nucleic acids research · 2021Article
- Characterization of the Aerobic Anoxygenic Phototrophic BacteriumMicroorganisms · 2021Article
- Transcriptional rewiring of the GcrA/CcrM bacterial epigenetic regulatory system in closely related bacteria.PLoS genetics · 2021Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation regulates many processes, including gene expression, by superimposing secondary information on DNA sequences. The conserved CcrM enzyme, which methylates adenines in GANTC sequences, is essential to the viability of several Alphaproteobacteria. In this study, we find that Caulobacter crescentus cells lacking the CcrM enzyme accumulate low levels of the two conserved FtsZ and MipZ proteins, leading to a severe defect in cell division. This defect can be compensated by the expression of the ftsZ gene from an inducible promoter or by spontaneous suppressor mutations that promote FtsZ accumulation. We show that CcrM promotes the transcription of the ftsZ and mipZ genes and that the ftsZ and mipZ promoter regions contain a conserved CGACTC motif that is critical to their activities and to their regulation by CcrM. In addition, our results suggest that the ftsZ promoter has the lowest activity when the CGACTC motif is non-methylated, an intermediate activity when it is hemi-methylated and the highest activity when it is fully methylated. The regulation of ftsZ expression by DNA methylation may explain why CcrM is essential in a subset of Alphaproteobacteria.
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