ArticleTransfusion2013
Red blood cells stored for increasing periods produce progressive impairments in nitric oxide-mediated vasodilation.
Article in Transfusion, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- B-type natriuretic peptide and plasma hemoglobin levels following transfusion of shorter-storage versus longer-storage red blood cells: Results from the TOTAL randomized trial.American heart journal · 2017Trial
- Effects of storage-aged red blood cell transfusions on endothelial function in hospitalized patients.Transfusion · 2015Trial
- Effects of plasma donation on blood rheology and peripheral vascular function.The Journal of physiology · 2026Article
- Donor- and unit-specific factors influencing hemolysis in stored canine and feline packed red blood cells.Frontiers in veterinary science · 2026Article
- Different-sized extracellular vesicles derived from stored red blood cells package diverse cargoes and cause distinct cellular effects.Transfusion · 2023Article
- The oxygen dissociation curve of blood in COVID-19-An update.Frontiers in medicine · 2023Review
- Review
- Red blood cell storage lesion: causes and potential clinical consequences.Blood transfusion = Trasfusione del sangue · 2019Review
- Role of heme in lung bacterial infection after trauma hemorrhage and stored red blood cell transfusion: A preclinical experimental study.PLoS medicine · 2018Article
- RBC Transfusions Are Associated With Prolonged Mechanical Ventilation in Pediatric Acute Respiratory Distress Syndrome.Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies · 2018Article
- Influence of red blood cell-derived microparticles upon vasoregulation.Blood transfusion = Trasfusione del sangue · 2017Review
- Red Blood Cell Storage Lesion-Induced Adverse Effects: More Smoke; Is There Fire?Anesthesia and analgesia · 2017Article
- Physiologic Impact of Circulating RBC Microparticles upon Blood-Vascular Interactions.Frontiers in physiology · 2017Review
- Multi-omics Evidence for Inheritance of Energy Pathways in Red Blood Cells.Molecular & cellular proteomics : MCP · 2016Article
- Testosterone-dependent sex differences in red blood cell hemolysis in storage, stress, and disease.Transfusion · 2016Article
- Predicting storage-dependent damage to red blood cells using nitrite oxidation kinetics, peroxiredoxin-2 oxidation, and hemoglobin and free heme measurements.Transfusion · 2015Article
- Red blood cell washing, nitrite therapy, and antiheme therapies prevent stored red blood cell toxicity after trauma-hemorrhage.Free radical biology & medicine · 2015Article
- Established and theoretical factors to consider in assessing the red cell storage lesion.Blood · 2015Article
- Peroxiredoxin-2 recycling is inhibited during erythrocyte storage.Antioxidants & redox signaling · 2015Article
- Transfusion related morbidity in premature babies: Possible mechanisms and implications for practice.World journal of clinical pediatrics · 2014Review
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8 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundClinical outcomes in transfused patients may be affected by the duration of blood storage, possibly due to red blood cell (RBC)-mediated disruption of nitric oxide (NO) signaling, a key regulator of vascular tone and blood flow. STUDY DESIGN AND
methodsAS-1 RBC units stored up to 42 days were sampled at selected storage times. Samples were added to aortic rings ex vivo, a system where NO-mediated vasodilation could be experimentally controlled.
resultsRBC units showed storage-dependent changes in plasma hemoglobin (Hb), RBC 2,3-diphosphoglycerate acid, and RBC adenosine triphosphate conforming to expected profiles. When freshly collected (Day 0) blood was added to rat aortic rings, methacholine (MCh) stimulated substantial NO-mediated vasodilation. In contrast, MCh produced no vasodilation in the presence of blood stored for 42 days. Surprisingly, the vasoinhibitory effects of stored RBCs were almost totally mediated by RBCs themselves: removal of the supernatant did not attenuate the inhibitory effects, while addition of supernatant alone to the aortic rings only minimally inhibited MCh-stimulated relaxation. Stored RBCs did not inhibit vasodilation by a direct NO donor, demonstrating that the RBC-mediated vasoinhibitory mechanism did not work by NO scavenging.
conclusionsThese studies have revealed a previously unrecognized vasoinhibitory activity of stored RBCs, which is more potent than the described effects of free Hb and works through a different mechanism that does not involve NO scavenging but may function by reducing endothelial NO production. Through this novel mechanism, transfusion of small volumes of stored blood may be able to disrupt physiologic vasodilatory responses and thereby possibly cause adverse clinical outcomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.