ArticleJournal of the American Chemical Society2013
Forming cross-linked peptidoglycan from synthetic gram-negative Lipid II.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 57 citations in OpenAlex.
- Lipid II unlocked: strategies for obtaining a major antibiotic target.Chemical communications (Cambridge, England) · 2025Review
- Phage protein Gp11 blocksmBio · 2024Article
- Protein secretion zones during overexpression of amylase within the Gram-positive cell wall.BMC biology · 2023Article
- Metabolic Processing of Selenium-Based Bioisosteres ofBiochemistry · 2022Article
- Impact of crossbridge structure on peptidoglycan crosslinking: A synthetic stem peptide approach.Methods in enzymology · 2022Article
- Real-time monitoring of peptidoglycan synthesis by membrane-reconstituted penicillin-binding proteins.eLife · 2021Article
- Structure and reconstitution of a hydrolase complex that may release peptidoglycan from the membrane after polymerization.Nature microbiology · 2021Article
- Remodeling of Cross-bridges Controls Peptidoglycan Cross-linking Levels in Bacterial Cell Walls.ACS chemical biology · 2020Article
- Uncovering the activities, biological roles, and regulation of bacterial cell wall hydrolases and tailoring enzymes.The Journal of biological chemistry · 2020Review
- Insight into Elongation Stages of Peptidoglycan Processing in Bacterial Cytoplasmic Membranes.Scientific reports · 2018Article
- Membrane Potential Is Required for MurJ Function.Journal of the American Chemical Society · 2018Article
- Substrate Preferences Establish the Order of Cell Wall Assembly in Staphylococcus aureus.Journal of the American Chemical Society · 2018Article
- Identification of a Functionally Unique Family of Penicillin-Binding Proteins.Journal of the American Chemical Society · 2017Article
- Peptidoglycan Cross-Linking Preferences of Staphylococcus aureus Penicillin-Binding Proteins Have Implications for Treating MRSA Infections.Journal of the American Chemical Society · 2017Article
- Lipid II overproduction allows direct assay of transpeptidase inhibition by β-lactams.Nature chemical biology · 2017Article
- In vitro reconstitution demonstrates the cell wall ligase activity of LCP proteins.Nature chemical biology · 2017Article
- Antimicrobial lipopeptide tridecaptin A1 selectively binds to Gram-negative lipid II.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Chemoenzymatic synthesis of the bacterial polysaccharide repeating unit undecaprenyl pyrophosphate and its analogs.Nature protocols · 2016Article
- Enantioselective synthesis of α-alkenyl α-amino acidsChemical science · 2016Article
- Chemoenzymatic syntheses of water-soluble lipid I fluorescent probes.Tetrahedron letters · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The bacterial cell wall precursor, Lipid II, has a highly conserved structure among different organisms except for differences in the amino acid sequence of the peptide side chain. Here, we report an efficient and flexible synthesis of the canonical Lipid II precursor required for the assembly of Gram-negative peptidoglycan (PG). We use a rapid LC/MS assay to analyze PG glycosyltransfer (PGT) and transpeptidase (TP) activities of Escherichia coli penicillin binding proteins PBP1A and PBP1B and show that the native m-DAP residue in the peptide side chain of Lipid II is required in order for TP-catalyzed peptide cross-linking to occur in vitro. Comparison of PG produced from synthetic canonical E. coli Lipid II with PG isolated from E. coli cells demonstrates that we can produce PG in vitro that resembles native structure. This work provides the tools necessary for reconstituting cell wall synthesis, an essential cellular process and major antibiotic target, in a purified system.
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