ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2013
Identification of a reductive tetrachloroethene dehalogenase in Shewanella sediminis.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 40 citations in OpenAlex.
- Genome-resolved transcriptomics reveals novel PCE-dehalogenating bacteria from Aarhus Bay sediments.mSystems · 2025Article
- Deciphering reductive dehalogenase specificity through targeted mutagenesis of chloroalkane reductases.Applied and environmental microbiology · 2025Article
- Toward the development of a molecular toolkit for the microbial remediation of per-and polyfluoroalkyl substances.Applied and environmental microbiology · 2024Review
- Catabolic Reductive Dehalogenase Substrate Complex Structures Underpin Rational Repurposing of Substrate Scope.Microorganisms · 2020Article
- Biological synthesis of iron nanoparticles using hydrolysates from a waste-based biorefinery.Environmental science and pollution research international · 2020Article
- Dehalogenases: From Improved Performance to Potential Microbial Dehalogenation Applications.Molecules (Basel, Switzerland) · 2018Review
- Microbial degradation of chloroethenes: a review.Environmental science and pollution research international · 2017Review
- Bacterial communities associated with anaerobic debromination of decabromodiphenyl ether from mangrove sediment.Environmental science and pollution research international · 2017Article
- Novel Firmicutes group implicated in the dechlorination of two chlorinated xanthones, analogues of natural organochlorines.Applied and environmental microbiology · 2014Article
- High frequency of phylogenetically diverse reductive dehalogenase-homologous genes in deep subseafloor sedimentary metagenomes.Frontiers in microbiology · 2014Article
- Organohalide respiration: microbes breathing chlorinated molecules.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
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Abstract
The genome sequence of psychrophilic Shewanella sediminis revealed the presence of five putative reductive dehalogenases (Rdhs). We found that cell extracts of pyruvate/fumarate-grown S. sediminis cells catalysed reduced methyl viologen-dependent reductive dechlorination of tetrachloroethene (PCE) to trichloroethene (TCE) at a specific activity of approximately 1 nmol TCE min(-1) (mg protein)(-1). Dechlorination of PCE followed Michaelis-Menten kinetics with an apparent Km of 120 μM PCE. No PCE dechlorination was observed with heat-denatured extract or when cyanocobalamin was omitted from the growth medium; however, the presence of PCE in the growth medium increased PCE transformation rates. Analysis of mutants carrying in-frame deletions of all five Rdhs encoding genes showed that only deletion of Ssed_3769 resulted in the loss of PCE dechlorination activity suggesting that Ssed_3769 is a functional Rdh. This is the first study to show reductive dechlorination activity of PCE in a sediment-dwelling Shewanella species that may be important for linking the flux of organohalogens to organic carbon via reductive dehalogenation in marine sediments.
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