ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2013
Organic cofactors in the metabolism of Dehalococcoides mccartyi strains.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.
- Meta-analyses of Dehalococcoides mccartyi strain 195 transcriptomic profiles identify a respiration rate-related gene expression transition point and interoperon recruitment of a key oxidoreductase subunit.Applied and environmental microbiology · 2014Pooled it
- Environmental considerations and bioremediation applications of the metal-, nitrate-, and organohalide-respiring bacteriumApplied and environmental microbiology · 2026Review
- The Effects of Biochar on the Revival and Performance of an Organohalide-Respiring Mixed Culture.Environmental science & technology · 2026Article
- Reductive dehalogenase ofFrontiers in microbiology · 2024Article
- Quest for Nitrous Oxide-reducing Bacteria Present in an Anammox Biofilm Fed with Nitrous Oxide.Microbes and environments · 2024Article
- Structure of a membrane-bound menaquinol:organohalide oxidoreductase.Nature communications · 2023Article
- Taxonomic Re-Classification and Expansion of the Phylum Chloroflexota Based on over 5000 Genomes and Metagenome-Assembled Genomes.Microorganisms · 2023Article
- Article
- Combined read- and assembly-based metagenomics to reconstruct a Dehalococcoides mccartyi genome from PCB-contaminated sediments and evaluate functional differences among organohalide-respiring consortia in the presence of different halogenated contaminants.FEMS microbiology ecology · 2022Article
- Interspecies metabolite transfer and aggregate formation in a co-culture of Dehalococcoides and Sulfurospirillum dehalogenating tetrachloroethene to ethene.The ISME journal · 2021Article
- Genome Sequence, Proteome Profile, and Identification of a Multiprotein Reductive Dehalogenase Complex inJournal of proteome research · 2021Article
- Metagenomic Analysis Reveals Microbial Interactions at the Biocathode of a Bioelectrochemical System Capable of Simultaneous Trichloroethylene and Cr(VI) Reduction.Frontiers in microbiology · 2021Article
- Iron Sulfide Enhanced the Dechlorination of Trichloroethene byFrontiers in microbiology · 2021Article
- Identification and characterization of a bacterial core methionine synthase.Scientific reports · 2020Article
- Targeted detection of Dehalococcoides mccartyi microbial protein biomarkers as indicators of reductive dechlorination activity in contaminated groundwater.Scientific reports · 2019Article
- Detection of Organohalide-Respiring Enzyme Biomarkers at a Bioaugmented TCE-Contaminated Field Site.Frontiers in microbiology · 2019Article
- Insights Into the Redox Sensitivity ofFrontiers in microbiology · 2018Article
- Metagenomic and Metatranscriptomic Analyses Reveal the Structure and Dynamics of a Dechlorinating Community Containing Dehalococcoides mccartyi and Corrinoid-Providing Microorganisms under Cobalamin-Limited Conditions.Applied and environmental microbiology · 2017Article
- Identification of specific corrinoids reveals corrinoid modification in dechlorinating microbial communities.Environmental microbiology · 2015Article
- Efficient metabolic exchange and electron transfer within a syntrophic trichloroethene-degrading coculture of Dehalococcoides mccartyi 195 and Syntrophomonas wolfei.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dehalococcoides mccartyi strains are strictly anaerobic organisms specialized to grow with halogenated compounds as electron acceptor via a respiratory process. Their genomes are among the smallest known for free-living organisms, and the embedded gene set reflects their strong specialization. Here, we briefly review main characteristics of published Dehalococcoides genomes and show how genome information together with cultivation and biochemical experiments have contributed to our understanding of Dehalococcoides physiology and biochemistry. We extend this approach by the detailed analysis of cofactor metabolism in Dehalococcoides strain CBDB1. Dehalococcoides genomes were screened for encoded proteins annotated to contain or interact with organic cofactors, and the expression of these proteins was analysed by shotgun proteomics to shed light on cofactor requirements. In parallel, cultivation experiments testing for vitamin requirements showed that cyanocobalamin (vitamin B12), thiamine and biotin were essential supplements and that cyanocobalamin could be substituted by dicyanocobinamide and dimethylbenzimidazole. Dehalococcoides genome analysis, detection of single enzymes by shotgun proteomics and inhibition studies confirmed the expression of the biosynthetic pathways for pyridoxal-5-phosphate, flavin nucleotides, folate, S-adenosylmethionine, pantothenate and nicotinic acids in strain CBDB1. Haem/cytochromes, quinones and lipoic acids were not necessary for cultivation or dechlorination activity and no biosynthetic pathways were identified in the genomes.
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