ArticleThe Journal of biological chemistry2013
Inorganic pyrophosphatase defects lead to cell cycle arrest and autophagic cell death through NAD+ depletion in fermenting yeast.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 75 citations in OpenAlex.
- Article
- PPa1 insufficiency drives lysosomal storage disease and inflammatory macrophage expansion in the bone marrow.bioRxiv : the preprint server for biology · 2026Article
- PPA1 promotes oxidative phosphorylation and malignant progression of colorectal cancer under glucose restriction via AMPK/ULK1/FUNDC1-mediated mitophagy.Cell death discovery · 2025Article
- Engineering Inorganic Pyrophosphate Metabolism as a Strategy to Generate a Fluoride-ResistantMicroorganisms · 2025Article
- Evaluation of Pyrophosphate-Driven Proton Pumps inMicroorganisms · 2024Article
- Review
- Genome-scale metabolic model analysis of Pichia pastoris for enhancing the production of S-adenosyl-L-methionine.Bioprocess and biosystems engineering · 2023Article
- Article
- Specific Features of the Proteomic Response of Thermophilic BacteriumInternational journal of molecular sciences · 2022Article
- Article
- Essential role of pyrophosphate homeostasis mediated by the pyrophosphate-dependent phosphofructokinase in Toxoplasma gondii.PLoS pathogens · 2022Article
- PPA1, an energy metabolism initiator, plays an important role in the progression of malignant tumors.Frontiers in oncology · 2022Review
- Morphological characteristics and transcriptome analysis at different anther development stages of the male sterile mutant MS7-2 in Wucai (Brassica campestris L.).BMC genomics · 2021Article
- Crystal Structure of Inorganic Pyrophosphatase FromFrontiers in cell and developmental biology · 2021Article
- The HFrontiers in plant science · 2020Article
- Phosphate Homeostasis - A Vital Metabolic Equilibrium Maintained Through the INPHORS Signaling Pathway.Frontiers in microbiology · 2020Review
- Lack of Vacuolar HFrontiers in plant science · 2020Article
- Article
- Vacuolar Proton Pyrophosphatase Is Required for High Magnesium Tolerance inInternational journal of molecular sciences · 2018Article
- Vacuolar HThe Plant cell · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inorganic pyrophosphatases are required for anabolism to take place in all living organisms. Defects in genes encoding these hydrolytic enzymes are considered inviable, although their exact nature has not been studied at the cellular and molecular physiology levels. Using a conditional mutant in IPP1, the Saccharomyces cerevisiae gene encoding the cytosolic soluble pyrophosphatase, we show that respiring cells arrest in S phase upon Ipp1p deficiency, but they remain viable and resume growth if accumulated pyrophosphate is removed. However, fermenting cells arrest in G1/G0 phase and suffer massive vacuolization and eventual cell death by autophagy. Impaired NAD(+) metabolism is a major determinant of cell death in this scenario because demise can be avoided under conditions favoring accumulation of the oxidized pyridine coenzyme. These results posit that the mechanisms related to excess pyrophosphate toxicity in eukaryotes are dependent on the energy metabolism of the cell.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.