ArticleProceedings of the National Academy of Sciences of the United States of America2013
Binding of Drosophila Polo kinase to its regulator Matrimony is noncanonical and involves two separate functional domains.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 31 citations in OpenAlex.
- Meiosis-specific functions of kinetochore protein SPC105R required for chromosome segregation inMolecular biology of the cell · 2024Article
- Meiosis-specific functions of kinetochore protein SPC105R required for chromosome segregation inbioRxiv : the preprint server for biology · 2024Article
- B chromosomes reveal a female meiotic drive suppression system in Drosophila melanogaster.Current biology : CB · 2023Article
- Regulation of oocyte maturation: Role of conserved ERK signaling.Molecular reproduction and development · 2022Review
- CEP97 phosphorylation by Dyrk1a is critical for centriole separation during multiciliogenesis.The Journal of cell biology · 2022Article
- Targeting Polo-like kinase in space and time during C. elegans meiosis.Cell cycle (Georgetown, Tex.) · 2021Review
- Exo1 recruits Cdc5 polo kinase to MutLγ to ensure efficient meiotic crossover formation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Meiosis I Kinase Regulators: Conserved Orchestrators of Reductional Chromosome Segregation.BioEssays : news and reviews in molecular, cellular and developmental biology · 2020Article
- The E3 ubiquitin ligase Sina regulates the assembly and disassembly of the synaptonemal complex in Drosophila females.PLoS genetics · 2019Article
- Functional Consequences of the Evolution of Matrimony, a Meiosis-Specific Inhibitor of Polo Kinase.Molecular biology and evolution · 2019Article
- Article
- B Chromosomes in theGenes · 2018Review
- Female Meiosis: Synapsis, Recombination, and Segregation inGenetics · 2018Review
- Spindle Assembly and Chromosome Segregation Requires Central Spindle Proteins in Drosophila Oocytes.Genetics · 2016Article
- Article
- Understanding the Polo Kinase machine.Oncogene · 2015Review
- Autoinhibition and relief mechanism for Polo-like kinase 4.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A Drosophila model identifies a critical role for zinc in mineralization for kidney stone disease.PloS one · 2015Article
- Topoisomerase II is required for the proper separation of heterochromatic regions during Drosophila melanogaster female meiosis.PLoS genetics · 2014Article
- Polo-like kinases: structural variations lead to multiple functions.Nature reviews. Molecular cell biology · 2014Review
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15 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drosophila melanogaster Polo kinase physically interacts with, and is repressed by, the Matrimony (Mtrm) protein during oogenesis. Females heterozygous for a deletion of the mtrm gene display defects in chromosome segregation at meiosis I. However, a complete absence of Mtrm results in both meiotic catastrophe and female sterility. We show that three phosphorylated residues in an N-terminal region in Mtrm are required for Mtrm::Polo binding. However, this binding is noncanonical; it does not require either a complete S-pS/pT-P motif in Mtrm or key residues in the Polo-box domain of Polo that allow Polo to bind phosphorylated substrates. By using fluorescence cross-correlation spectroscopy to characterize the Mtrm::Polo interaction in vivo, we show that a sterile α-motif (SAM) domain located at the C terminus of Mtrm increases the stability of Mtrm::Polo binding. Although Mtrm's C-terminal SAM domain is not required to rescue the chromosome segregation defects observed in mtrm/+ females, it is essential to prevent both meiotic catastrophe and the female sterility observed in mtrm/mtrm females. We propose that Polo's interaction with the cluster of phosphorylated residues alone is sufficient to rescue the meiosis I defect. However, the strengthening of Mtrm::Polo binding mediated by the SAM domain is necessary to prevent meiotic catastrophe and ensure female fertility. Characterization of the Mtrm::Polo interaction, as well as that of other Polo regulators, may assist in the design of a new class of Polo inhibitors to be used as targeted anticancer therapeutic agents.
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