ArticleProceedings of the National Academy of Sciences of the United States of America2013
Microfluidic processor allows rapid HER2 immunohistochemistry of breast carcinomas and significantly reduces ambiguous (2+) read-outs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed.
- Dual-mode microfluidic immunostaining device for diagnostic biomarkers detection and tumor microenvironment evaluation.Science advances · 2026Article
- Spatial-Omics Methods and Applications.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Fully automated sequential immunofluorescence (seqIF) for hyperplex spatial proteomics.Scientific reports · 2023Article
- A multi-depth spiral milli fluidic device for whole mount zebrafish antibody staining.Biomedical microdevices · 2023Article
- The VersaLive platform enables microfluidic mammalian cell culture for versatile applications.Communications biology · 2022Article
- Microfluidic Device for On-Chip Immunophenotyping and Cytogenetic Analysis of Rare Biological Cells.Cells · 2020Article
- Rapid micro-immunohistochemistry.Microsystems & nanoengineering · 2020Article
- A versatile microfluidic device for multiple ex vivo/in vitro tissue assays unrestrained from tissue topography.Microsystems & nanoengineering · 2020Article
- Microfluidic-based immunohistochemistry for breast cancer diagnosis: a comparative clinical study.Virchows Archiv : an international journal of pathology · 2019Article
- On-chip immunofluorescence analysis of single cervical cells using an electroactive microwell array with barrier for cervical screening.Biomicrofluidics · 2019Article
- High-content, cell-by-cell assessment of HER2 overexpression and amplification: a tool for intratumoral heterogeneity detection in breast cancer.Laboratory investigation; a journal of technical methods and pathology · 2019Article
- Ultra-fast and automated immunohistofluorescent multistaining using a microfluidic tissue processor.Scientific reports · 2019Article
- Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections.Microsystems & nanoengineering · 2019Article
- Combining fluorescence-based image segmentation and automated microfluidics for ultrafast cell-by-cell assessment of biomarkers for HER2-type breast carcinoma.Journal of biomedical optics · 2018Article
- Microfluidics-based immunofluorescence for fast staining of ALK in lung adenocarcinoma.Diagnostic pathology · 2018Article
- Microfluidic on-chip immunohistochemistry directly from a paraffin-embedded section.Biomicrofluidics · 2018Article
- Microfluidics for rapid cytokeratin immunohistochemical staining in frozen sections.Laboratory investigation; a journal of technical methods and pathology · 2017Article
- A Microfluidic Immunostaining System Enables Quality Assured and Standardized Immunohistochemical Biomarker Analysis.Scientific reports · 2017Article
- Tissue lithography: Microscale dewaxing to enable retrospective studies on formalin-fixed paraffin-embedded (FFPE) tissue sections.PloS one · 2017Article
- Antigen Masking During Fixation and Embedding, Dissected.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Biomarker analysis is playing an essential role in cancer diagnosis, prognosis, and prediction. Quantitative assessment of immunohistochemical biomarker expression on tumor tissues is of clinical relevance when deciding targeted treatments for cancer patients. Here, we report a microfluidic tissue processor that permits accurate quantification of the expression of biomarkers on tissue sections, enabled by the ultra-rapid and uniform fluidic exchange of the device. An important clinical biomarker for invasive breast cancer is human epidermal growth factor receptor 2 [(HER2), also known as neu], a transmembrane tyrosine kinase that connotes adverse prognostic information for the patients concerned and serves as a target for personalized treatment using the humanized antibody trastuzumab. Unfortunately, when using state-of-the-art methods, the intensity of an immunohistochemical signal is not proportional to the extent of biomarker expression, causing ambiguous outcomes. Using our device, we performed tests on 76 invasive breast carcinoma cases expressing various levels of HER2. We eliminated more than 90% of the ambiguous results (n = 27), correctly assigning cases to the amplification status as assessed by in situ hybridization controls, whereas the concordance for HER2-negative (n = 31) and -positive (n = 18) cases was 100%. Our results demonstrate the clinical potential of microfluidics for accurate biomarker expression analysis. We anticipate our technique will be a diagnostic tool that will provide better and more reliable data, onto which future treatment regimes can be based.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.