ArticleProceedings of the National Academy of Sciences of the United States of America2013
Steroid-based facial amphiphiles for stabilization and crystallization of membrane proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 133 citations in OpenAlex.
- Improved Pendant-Bearing Glucose-Neopentyl Glycols for Membrane Protein Stability.Bioconjugate chemistry · 2025Article
- Structural and biophysical analysis of cytochrome P450 2C9*14 and *27 variants in complex with losartan.Journal of inorganic biochemistry · 2024Article
- Facade-Based Bicelles as a New Tool for Production of Active Membrane Proteins in a Cell-Free System.International journal of molecular sciences · 2023Article
- Strategies for the Purification of Membrane Proteins.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Mechanisms of membrane protein crystallization in 'bicelles'.Scientific reports · 2022Article
- Development of 1,3-acetonedicarboxylate-derived glucoside amphiphiles (ACAs) for membrane protein study.Chemical science · 2022Article
- Scrutinizing the Feasibility of Nonionic Surfactants to Form Isotropic Bicelles of Curcumin: a Potential Antiviral Candidate Against COVID-19.AAPS PharmSciTech · 2021Article
- Insights into the Genetic Variations of Human Cytochrome P450 2C9: Structural Analysis, Characterization and Comparison.International journal of molecular sciences · 2021Article
- Catalytically Cleavable Detergent for Membrane Protein Studies.ACS omega · 2021Article
- The Det.Belt Server: A Tool to Visualize and Estimate Amphipathic Solvent Belts around Membrane Proteins.Membranes · 2021Article
- Isolation and thermal stabilization of mouse ferroportin.FEBS open bio · 2021Article
- Diastereomeric Cyclopentane-Based Maltosides (CPMs) as Tools for Membrane Protein Study.Journal of the American Chemical Society · 2020Article
- Structure of Cytochrome P450 2C9*2 in Complex with Losartan: Insights into the Effect of Genetic Polymorphism.Molecular pharmacology · 2020Article
- Formulation of Bicelles Based on Lecithin-Nonionic Surfactant Mixtures.Materials (Basel, Switzerland) · 2020Article
- New Malonate-Derived Tetraglucoside Detergents for Membrane Protein Stability.ACS chemical biology · 2020Article
- Article
- So many roads traveled: A career in science and administration.The Journal of biological chemistry · 2020Article
- Probing thermostability of detergent-solubilized CBThe Journal of biological chemistry · 2020Article
- 1,3,5-Triazine-Cored Maltoside Amphiphiles for Membrane Protein Extraction and Stabilization.Journal of the American Chemical Society · 2019Article
- Stabilization of Human Multidrug Resistance Protein 4 (MRP4/ABCC4) Using Novel Solubilization Agents.SLAS discovery : advancing life sciences R & D · 2019Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
Abstract
Amphiphile selection is a critical step for structural studies of membrane proteins (MPs). We have developed a family of steroid-based facial amphiphiles (FAs) that are structurally distinct from conventional detergents and previously developed FAs. The unique FAs stabilize MPs and form relatively small protein-detergent complexes (PDCs), a property considered favorable for MP crystallization. We attempted to crystallize several MPs belonging to different protein families, including the human gap junction channel protein connexin 26, the ATP binding cassette transporter MsbA, the seven-transmembrane G protein-coupled receptor-like bacteriorhodopsin, and cytochrome P450s (peripheral MPs). Using FAs alone or mixed with other detergents or lipids, we obtained 3D crystals of the above proteins suitable for X-ray crystallographic analysis. The fact that FAs enhance MP crystallizability compared with traditional detergents can be attributed to several properties, including increased protein stability, formation of small PDCs, decreased PDC surface flexibility, and potential to mediate crystal lattice contacts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.