ArticleProceedings of the National Academy of Sciences of the United States of America2013
Homologs of vertebrate Opn3 potentially serve as a light sensor in nonphotoreceptive tissue.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed, 195 citations in OpenAlex.
- From pineal photoreception to optogenetics: functional colour opponency based on a pineal bistable opsin.Eye and vision (London, England) · 2026Review
- Dragonfly red opsins share a common tuning mechanism with mammalian red opsins and further enhancement of near-infrared sensitivity.Cellular and molecular life sciences : CMLS · 2026Article
- Non-visual opsins and their role in circadian photoentrainment.Eye and vision (London, England) · 2026Review
- The role and signal pathways of opsin 3 in the skin: from light perception to pathophysiological mechanisms.Frontiers in medicine · 2026Review
- G-protein-coupled receptor diversity and evolution in the closest living relatives of metazoa.eLife · 2025Article
- The physiological functions of OPN5m.Eye and vision (London, England) · 2025Review
- Validation of phiC31-mediated expression and functional knockout of Opn3 in the Opn3-phiC31o knock-in mouse.Eye and vision (London, England) · 2025Article
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- OPN3-mediated positive regulation of angiogenesis in HUVECs through VEGFR2 interaction.Communications biology · 2025Article
- An Extensive Survey of Vertebrate-specific, Nonvisual Opsins Identifies a Novel Subfamily, Q113-Bistable Opsin.Genome biology and evolution · 2025Article
- Hypothalamic opsin 3 suppresses MC4R signaling and potentiates Kir7.1 to promote food consumption.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Expression of opsin and visual cycle-related enzymes in fetal rat skin keratinocytes and cellular response to blue light.Biochemistry and biophysics reports · 2024Article
- Light-responsive adipose-hypothalamus axis controls metabolic regulation.Nature communications · 2024Article
- In-silico predicted mouse melanopsins with blue spectral shifts deliver efficient subcellular signaling.Cell communication and signaling : CCS · 2024Article
- Blue light irradiation inhibits the M2 polarization of the cancer-associated macrophages in colon cancer.BMC cancer · 2024Article
- Male rat hypothalamic extraretinal photoreceptor Opsin3 is sensitive to osmotic stimuli and light.Journal of neuroendocrinology · 2024Article
- Functional characterization of four opsins and two G alpha subtypes co-expressed in the molluscan rhabdomeric photoreceptor.BMC biology · 2023Article
- Optical Approaches for Investigating Neuromodulation and G Protein-Coupled Receptor Signaling.Pharmacological reviews · 2023Review
- Optogenetic manipulation of Gq- and Gi/o-coupled receptor signaling in neurons and heart muscle cells.eLife · 2023Article
26 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most opsins selectively bind 11-cis retinal as a chromophore to form a photosensitive pigment, which underlies various physiological functions, such as vision and circadian photoentrainment. Recently, opsin 3 (Opn3), originally called encephalopsin or panopsin, and its homologs were identified in various tissues including brain, eye, and liver in both vertebrates and invertebrates, including human. Because Opn3s are mainly expressed in tissues that are not considered to contain sufficient amounts of 11-cis retinal to form pigments, the photopigment formation ability of Opn3 has been of interest. Here, we report the successful expression of Opn3 homologs, pufferfish teleost multiple tissue opsin (PufTMT) and mosquito Opn3 (MosOpn3) and show that these proteins formed functional photopigments with 11-cis and 9-cis retinals. The PufTMT- and MosOpn3-based pigments have absorption maxima in the blue-to-green region and exhibit a bistable nature. These Opn3 homolog-based pigments activate Gi-type and Go-type G proteins light dependently, indicating that they potentially serve as light-sensitive Gi/Go-coupled receptors. We also demonstrated that mammalian cultured cells transfected with the MosOpn3 or PufTMT became light sensitive without the addition of 11-cis retinal and the photosensitivity retained after the continuous light exposure, showing a reusable pigment formation with retinal endogenously contained in culture medium. Interestingly, we found that the MosOpn3 also acts as a light sensor when constituted with 13-cis retinal, a ubiquitously present retinal isomer. Our findings suggest that homologs of vertebrate Opn3 might function as photoreceptors in various tissues; furthermore, these Opn3s, particularly the mosquito homolog, could provide a promising optogenetic tool for regulating cAMP-related G protein-coupled receptor signalings.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.