ArticleProceedings of the National Academy of Sciences of the United States of America2013
Cell-free study of F plasmid partition provides evidence for cargo transport by a diffusion-ratchet mechanism.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed, 149 citations in OpenAlex.
- The kinetics and mobility of a ParA ATPase drive carboxysome distribution inbioRxiv : the preprint server for biology · 2026Article
- Molecular basis of ParA ATPase activation by the CTPase ParB during bacterial chromosome segregation.Nature communications · 2025Article
- Brownian ratchet mechanisms for carboxysome positioning in bacteria.Current opinion in microbiology · 2025Review
- Expanding the diversity of bacterial DNA partitioning: A CTP-independent ParABProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Mutational analysis of the F plasmid partitioning protein ParA reveals residues required for oligomerization and plasmid maintenance.Nucleic acids research · 2025Article
- Carboxysome Shell Protein CcmK2 Assembles into Monodisperse and pH-Reversible Microparticles.ACS nano · 2025Article
- Three factors ParA, TipN, and DnaA-mediated chromosome replication initiation are contributors of centromere segregation inMolecular biology of the cell · 2024Article
- Plasmid partitioning driven by collective migration of ParA between nucleoid lobes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Connecting the dots: key insights on ParB for chromosome segregation from single-molecule studies.FEMS microbiology reviews · 2024Review
- TipN's involvement with centromere segregation in Caulobacter crescentus.bioRxiv : the preprint server for biology · 2023Article
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- VirB, a transcriptional activator of virulence in Shigella flexneri, uses CTP as a cofactor.Communications biology · 2023Article
- Kinetic principles of ParA2-ATP cycling guide dynamic subcellular localizations in Vibrio cholerae.Nucleic acids research · 2023Article
- Diverse Partners of the Partitioning ParB Protein in Pseudomonas aeruginosa.Microbiology spectrum · 2023Article
- A new role for monomeric ParA/Soj in chromosome dynamics in Bacillus subtilis.MicrobiologyOpen · 2023Article
- Polyploidy, regular patterning of genome copies, and unusual control of DNA partitioning in the Lyme disease spirochete.Nature communications · 2022Article
- High-throughput imaging and quantitative analysis uncovers the nature of plasmid positioning by ParABS.eLife · 2022Article
- Chromosome remodelling by SMC/Condensin inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Regulation of DNA replication initiation by ParA is independent ofMicrobiology (Reading, England) · 2022Article
- Distinct architectural requirements for theeLife · 2022Article
27 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Increasingly diverse types of cargo are being found to be segregated and positioned by ParA-type ATPases. Several minimalistic systems described in bacteria are self-organizing and are known to affect the transport of plasmids, protein machineries, and chromosomal loci. One well-studied model is the F plasmid partition system, SopABC. In vivo, SopA ATPase forms dynamic patterns on the nucleoid in the presence of the ATPase stimulator, SopB, which binds to the sopC site on the plasmid, demarcating it as the cargo. To understand the relationship between nucleoid patterning and plasmid transport, we established a cell-free system to study plasmid partition reactions in a DNA-carpeted flowcell. We observed depletion zones of the partition ATPase on the DNA carpet surrounding partition complexes. The findings favor a diffusion-ratchet model for plasmid motion whereby partition complexes create an ATPase concentration gradient and then climb up this gradient toward higher concentrations of the ATPase. Here, we report on the dynamic properties of the Sop system on a DNA-carpet substrate, which further support the proposed diffusion-ratchet mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.