ArticleJournal of biomedical optics2013
Advantages of full spectrum flow cytometry.
Article in Journal of biomedical optics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Guidelines for Gating Flow Cytometry Data for Immunological Assays.Methods in molecular biology (Clifton, N.J.) · 2027Article
- A Survey on Core Flow Cytometry Facilities: Instrument Maintenance, Usage, and Funding.Journal of biomolecular techniques : JBT · 2023Article
- Development of Spectral Imaging Cytometry.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Using Virtual Filtering Approach to Discriminate Microalgae by Spectral Flow Cytometer.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Advanced immunophenotyping: A powerful tool for immune profiling, drug screening, and a personalized treatment approach.Frontiers in immunology · 2023Article
- Flow Cytometry Detection of Anthracycline-Treated Breast Cancer Cells: An Optimized Protocol.Current issues in molecular biology · 2022Article
- Full spectrum flow cytometry and mass cytometry: A 32-marker panel comparison.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2022Article
- The Evolution of Single-Cell Analysis and Utility in Drug Development.The AAPS journal · 2021Review
- T Lymphocytes and Testicular Immunity: A New Insight into Immune Regulation in Testes.International journal of molecular sciences · 2020Review
- OMIP-069: Forty-Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020Article
- Novel full-spectral flow cytometry with multiple spectrally-adjacent fluorescent proteins and fluorochromes and visualization of in vivo cellular movement.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2015Article
- Single-cell technologies for monitoring immune systems.Nature immunology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A charge coupled device-based flow-cytometer for the measurement of full spectra was implemented and characterized. The spectral resolution was better than 1.5 nm and the coefficient of variation for fluorescence from flow check beads was 5% or better. Both cell and bead data were analyzed by fitting to measured component spectra. Separation of flow check and align flow beads, which have similar spectra, was nearly identical whether using a spectral analysis or a scatter analysis. After mixing, cells stained with ethidium bromide or propidium iodide were measured at different timepoints. The contribution of these 12 nm separated emission spectra could be separately quantified and the kinetic process of the samples becoming homogeneous due to fluorophor dissociation and rebinding was observed. Principle component analysis was used to reduce noise and alternating least squares (ALS) was used to analyze one set of noise-reduced cell data without knowledge of the component spectra. The component spectra obtained via ALS are very similar to the measured component spectra. The contributions of ethidium bromide and propidium iodide to the individual spectra are also similar to those obtained via the spectral fitting procedure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.