ArticleThe American journal of tropical medicine and hygiene2013
Whole-blood nested-PCR amplification of M. leprae-specific DNA for early diagnosis of leprosy.
Article in The American journal of tropical medicine and hygiene, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Accuracy of Ultrasound-Guided Ulnar Perineural Subcutaneous Aspirate for the Diagnosis and Control of Leprosy: Protocol for a Prospective Diagnostic Evaluation Study.JMIR research protocols · 2026Article
- Molecular Detection ofTropical medicine and infectious disease · 2025Review
- Polymorphisms in mitochondrial ribosomal protein S5 (MRPS5) are associated with leprosy risk in Chinese.PLoS neglected tropical diseases · 2020Article
- Leprosy in a low-incidence setting : Case report relevant to metagenomic next generation sequencing applications.Wiener klinische Wochenschrift · 2020Article
- Evaluation of antigen-specific immune responses for leprosy diagnosis in a hyperendemic area in China.PLoS neglected tropical diseases · 2018Article
- Accuracy of Enzyme-Linked Immunosorbent Assays (ELISAs) in Detecting Antibodies againstThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2018Review
- A Macrophage Response to Mycobacterium leprae Phenolic Glycolipid Initiates Nerve Damage in Leprosy.Cell · 2017Article
- Widespread nasal carriage of Mycobacterium lepraeamong a healthy population in a hyperendemic region of northeastern Brazil.Memorias do Instituto Oswaldo Cruz · 2015Article
- Asymptomatic Leprosy Infection among Blood Donors May Predict Disease Development and Suggests a Potential Mode of Transmission.Journal of clinical microbiology · 2015Article
- PCR-based techniques for leprosy diagnosis: from the laboratory to the clinic.PLoS neglected tropical diseases · 2014Review
- Application of RLEP real-time PCR for detection of M. leprae DNA in paraffin-embedded skin biopsy specimens for diagnosis of paucibacillary leprosy.The American journal of tropical medicine and hygiene · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We evaluated the sensitivity and specificity of a nested-polymerase chain reaction (PCR) method for detection of Mycobacterium leprae DNA from whole blood. Whole-blood specimens were subjected to nested-PCR amplification of M. leprae repeat DNA sequences in 49 multibacillary (MB) and 30 paucibacillary (PB) leprosy patients, 96 household contacts (HHCs), 18 tuberculosis (TB) patients, and 35 normal healthy individuals. M. leprae DNA was detected in 95.92% (47/49) of MB, 70% (21/30) of PB, and 6.25% (6/96) of HHC, but it was not detected in 18 TB or 35 normal controls. The sensitivities of the anti-bovine serum albumin (ND-O-BSA) immunoglobulin M (IgM) and antifusion protein of ML0405-ML2331 IgG for MB were 97.96% and 89.8%, and these values for PB were 70% and 53.33%. However, the ND-O-BSA enzyme-linked immunosorbent assay (ELISA) had lower specificity, with relatively high false-positive results for TB patients (16.67%) and normal healthy controls (10%). Based on these promising findings, we propose the use of nested PCR of whole-blood samples along with ELISA test for early detection of leprosy cases.
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