ArticleBioengineered
A single point mutation in the listerial betL σ(A)-dependent promoter leads to improved osmo- and chill-tolerance and a morphological shift at elevated osmolarity.
Article in Bioengineered. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Application of Whole Genome Sequencing to Aid in Deciphering the Persistence Potential ofMicroorganisms · 2021Review
- Molecular and Biochemical Characterization of Salt-Tolerant Trehalose-6-Phosphate Hydrolases Identified by Screening and Sequencing Salt-Tolerant Clones From the Metagenomic Library of the Gastrointestinal Tract.Frontiers in microbiology · 2020Article
- Functional metagenomics identifies novel genes ABCTPP, TMSRP1 and TLSRP1 among human gut enterotypes.Scientific reports · 2018Article
- Biosynthesis and uptake of glycine betaine as cold-stress response to low temperature in fish pathogen Vibrio anguillarum.Journal of microbiology (Seoul, Korea) · 2017Article
- Genotypes Associated withFrontiers in microbiology · 2017Article
- Shedding light on betL*: pPL2-lux mediated real-time analysis of betL* expression in Listeria monocytogenes.Bioengineered · 2016Article
- Designer probiotics: Development and applications in gastrointestinal health.World journal of gastrointestinal pathophysiology · 2015Review
- Under the microscope: From pathogens to probiotics and back.Bioengineered · 2015Review
- Metagenomics and novel gene discovery: promise and potential for novel therapeutics.Virulence · 2014Review
- Cronobacter sakazakii: stress survival and virulence potential in an opportunistic foodborne pathogen.Gut microbes · 2014Review
- Article
- Combined metagenomic and phenomic approaches identify a novel salt tolerance gene from the human gut microbiome.Frontiers in microbiology · 2014Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Betaine uptake in Listeria monocytogenes is mediated by three independent transport systems, the simplest of which in genetic terms is the secondary transporter BetL. Using a random mutagenesis approach, based on the E. coli XL1 Red mutator strain, we identified a single point mutation in a putative promoter region upstream of the BetL coding region which leads to a significant increase in betL transcript levels under osmo- and chill-stress conditions and a concomitant increase in stress tolerance. Furthermore, the mutation appears to counter the heretofore unreported "twisted" cell morphology observed for L. monocytogenes grown at elevated osmolarities in tryptone soy broth.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.