ArticleThe Journal of biological chemistry2013
Srb5/Med18-mediated termination of transcription is dependent on gene looping.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 47 citations in OpenAlex.
- The Mediator complex: co-transcriptional regulation and beyond.RNA biology · 2026Review
- Protein-interaction network analysis reveals the role of Prp19 splicing factor in transcription of both intron-containing and intron-lacking genes.PLoS genetics · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Growth-regulated co-occupancy of Mediator and Lsm3 at intronic ribosomal protein genes.Nucleic acids research · 2024Article
- The Mediator complex as a master regulator of transcription by RNA polymerase II.Nature reviews. Molecular cell biology · 2022Review
- Mediator Complex of the Malaria ParasiteACS omega · 2022Article
- Beyond the canonical role of TFIIB in eukaryotic transcription.Current genetics · 2022Review
- RNA polymerase II phosphorylation and gene looping: new roles for the Rpb4/7 heterodimer in regulating gene expression.Current genetics · 2020Review
- The role of Mediator and Little Elongation Complex in transcription termination.Nature communications · 2020Article
- RNA polymerase II plays an active role in the formation of gene loops through the Rpb4 subunit.Nucleic acids research · 2019Article
- Splicing and transcription touch base: co-transcriptional spliceosome assembly and function.Nature reviews. Molecular cell biology · 2017Review
- Genomewide Analysis of Clp1 Function in Transcription in Budding Yeast.Scientific reports · 2017Article
- Proteomic Analysis of the Mediator Complex Interactome in Saccharomyces cerevisiae.Scientific reports · 2017Article
- Mediator binding to UASs is broadly uncoupled from transcription and cooperative with TFIID recruitment to promoters.The EMBO journal · 2016Article
- Genome-wide analysis of chromatin packing in Arabidopsis thaliana at single-gene resolution.Genome research · 2016Article
- Enhancement of Transcription by a Splicing-Competent Intron Is Dependent on Promoter Directionality.PLoS genetics · 2016Article
- An Integrated Genomic Strategy Delineates Candidate Mediator Genes Regulating Grain Size and Weight in Rice.Scientific reports · 2016Article
- The point of no return: The poly(A)-associated elongation checkpoint.RNA biology · 2016Article
- Article
- Gene looping facilitates TFIIH kinase-mediated termination of transcription.Scientific reports · 2015Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have earlier demonstrated the involvement of Mediator subunit Srb5/Med18 in the termination of transcription for a subset of genes in yeast. Srb5/Med18 could affect termination either indirectly by modulating CTD-Ser(2) phosphorylation near the 3' end of a gene or directly by physically interacting with the cleavage and polyadenylation factor or cleavage factor 1 (CF1) complex and facilitating their recruitment to the terminator region. Here, we show that the CTD-Ser(2) phosphorylation pattern on Srb5/Med18-dependent genes remains unchanged in the absence of Srb5 in cells. Coimmunoprecipitation analysis revealed the physical interaction of Srb5/Med18 with the CF1 complex. No such interaction of Srb5/Med18 with the cleavage and polyadenylation factor complex, however, could be detected. The Srb5/Med18-CF1 interaction was not observed in the looping defective sua7-1 strain. Srb5/Med18 cross-linking to the 3' end of genes was also abolished in the sua7-1 strain. Chromosome conformation capture analysis revealed that the looped architecture of Srb5/Med18-dependent genes was abrogated in srb5(-) cells. Furthermore, Srb5-dependent termination of transcription was compromised in the looping defective sua7-1 cells. The overall conclusion of these results is that gene looping plays a crucial role in Srb5/Med18 facilitated termination of transcription, and the looped gene architecture may have a general role in termination of transcription in budding yeast.
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