ArticleJournal of bacteriology2013
The vitamin B₆ biosynthesis pathway in Streptococcus pneumoniae is controlled by pyridoxal 5'-phosphate and the transcription factor PdxR and has an impact on ear infection.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 64 citations in OpenAlex.
- Investigation of the effects of ampicillin and ceftazidime on Proteus mirabilis using metabolomic approaches and bioinformatics analyses.Folia microbiologica · 2026Article
- Cyclic Di-AMP Affects Cell Membrane Integrity of Streptococcus pneumoniae.Molecular microbiology · 2025Article
- Gene sco1417 encodes a positive regulator of the de novo biosynthesis of pyridoxal 5'-phosphate (vitamin B6) in Streptomyces coelicolor M145.BMC microbiology · 2025Article
- Revealing fitness and virulence determinants of hypervirulentFrontiers in cellular and infection microbiology · 2025Article
- Cronobacter sakazakii Pyridoxal Kinase PdxY Mediated by TreR and pESA3 Is Essential for Vitamin BApplied and environmental microbiology · 2023Article
- Structural insights into the DNA recognition mechanism by the bacterial transcription factor PdxR.Nucleic acids research · 2023Article
- Proteomic and Phenotypic Analyses of a Putative YggS Family Pyridoxal Phosphate-Dependent Enzyme in Acidovorax citrulli.The plant pathology journal · 2023Article
- Pyridoxal 5'-phosphate synthesis and salvage in Bacteria and Archaea: predicting pathway variant distributions and holes.Microbial genomics · 2023Article
- Degenerative Cervical Myelopathy induces sex-specific dysbiosis in mice.Frontiers in microbiology · 2023Article
- Pyridoxine biosynthesis protein MoPdx1 affects the development and pathogenicity ofFrontiers in cellular and infection microbiology · 2023Article
- Recombinant Expression, Purification and Characterization of Pyridoxal 5'-phosphate Synthase fromIranian journal of biotechnology · 2021Article
- Actinobacillus utilizes a binding protein-dependent ABC transporter to acquire the active form of vitamin BThe Journal of biological chemistry · 2021Article
- Knowns and Unknowns of Vitamin BEcoSal Plus · 2021Review
- Underground metabolism facilitates the evolution of novel pathways for vitamin B6 biosynthesis.Applied microbiology and biotechnology · 2021Review
- Bifidobacterium adolescentis as a key member of the human gut microbiota in the production of GABA.Scientific reports · 2020Article
- Essential Metabolic Routes as a Way to ESKAPE From Antibiotic Resistance.Frontiers in public health · 2020Article
- Conformational transitions induced by γ-amino butyrate binding in GabR, a bacterial transcriptional regulator.Scientific reports · 2019Article
- DdlR, an essential transcriptional regulator of peptidoglycan biosynthesis in Clostridioides difficile.Molecular microbiology · 2019Article
- A Survey of Pyridoxal 5'-Phosphate-Dependent Proteins in the Gram-Positive Model BacteriumFrontiers in molecular biosciences · 2019Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Vitamin B₆ is an essential cofactor for a large number of enzymes in both prokaryotes and eukaryotes. In this study, we characterized the pyridoxal 5'-phosphate (PLP) biosynthesis pathway in Streptococcus pneumoniae. Our results revealed that S. pneumoniae possesses a de novo vitamin B₆ biosynthesis pathway encoded by the pdxST genes. Purified PdxS functionally displayed as PLP synthase, whereas PdxT exhibited glutaminase activity in vitro. Deletion of pdxS, but not pdxT, resulted in a vitamin B₆ auxotrophic mutant. The defective growth of the ΔpdxS mutant in a vitamin B₆-depleted medium could be chemically restored in the presence of the B₆ vitamers at optimal concentrations. By analyzing PdxS expression levels, we demonstrated that the expression of pdxS was repressed by PLP and activated by a transcription factor, PdxR. A pneumococcal ΔpdxR mutant also exhibited as a vitamin B₆ auxotroph. In addition, we found that disruption of the vitamin B₆ biosynthesis pathway in S. pneumoniae caused a significant attenuation in a chinchilla middle ear infection model and a minor attenuation in a mouse pneumonia model, indicating that the impact of vitamin B₆ synthesis on virulence depends upon the bacterial infection niche.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.