ArticleJournal of bacteriology2013
SMC condensation centers in Bacillus subtilis are dynamic structures.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Chromosome segregation in a minimal bacterial cell driven by SMC protein complexes.Protein science : a publication of the Protein Society · 2026Article
- Machine learning classification of trajectories from molecular dynamics simulations of chromosome segregation.PloS one · 2022Article
- Mechanisms for Chromosome Segregation in Bacteria.Frontiers in microbiology · 2021Review
- Bacillus subtilis Smc condenses chromosomes in a heterologous cell system, which is down-regulated by ScpAB.BMC research notes · 2020Article
- Article
- Article
- Article
- Single molecule tracking reveals that the bacterial SMC complex moves slowly relative to the diffusion of the chromosome.Nucleic acids research · 2018Article
- Rapid turnover of DnaA at replication origin regions contributes to initiation control of DNA replication.PLoS genetics · 2017Article
- Pseudomonas aeruginosa Condensins Support Opposite Differentiation States.Journal of bacteriology · 2016Article
- Insights into ParB spreading from the complex structure of Spo0J and parS.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The bacterial chromosome: architecture and action of bacterial SMC and SMC-like complexes.FEMS microbiology reviews · 2014Review
- MukBEF, a chromosomal organizer.Journal of molecular microbiology and biotechnology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SMC and MukB complexes consist of a central SMC dimer and two essential binding partners, ScpA and ScpB (MukE and MukF), and are crucial for correct chromosome compaction and segregation. The complexes form two bipolar assemblies on the chromosome, one in each cell half. Using fluorescence recovery after photobleaching (FRAP), we provide evidence that the SMC complex has high exchange rates. This depends to a considerable degree on de novo protein synthesis, revealing that the bacterial SMC complex has high on and off rates for binding to the chromosome. A mutation in SMC that affects ATPase activity and results in exaggerated DNA binding in vitro causes a strong segregation defect in vivo and affects the localization of the entire SMC complex, which localizes to many more sites in the cell than under normal conditions. These data indicate that ATP turnover is important for the function of Bacillus subtilis SMC. In contrast, the centromere protein Spo0J and DNA gyrase showed much less exchange between distinct binding sites on the chromosome than that seen with SMC. Binding of Spo0J to the origin regions was rather static and remained partially conserved until the next cell cycle. Our experiments reveal that the SMC complex has a high, condensin-like turnover rate and that an alteration of the ATPase cycle affects SMC function in vivo, while several nucleoid-associated proteins feature limited or slow exchange between different sites on the nucleoid, which may be the basis for epigenetic-like phenomena observed in bacteria.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.