ArticleEukaryotic cell2013
Nuclear pores and perinuclear expression sites of var and ribosomal DNA genes correspond to physically distinct regions in Plasmodium falciparum.
Article in Eukaryotic cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 34 citations in OpenAlex.
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- Cellular Hallmarks From Volume Electron Microscopy Reveal Developmental Progression of Plasmodium Ookinetes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
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- Comparative analysis of peripheral whole blood transcriptome from asymptomatic carriers reveals upregulation of subsets of surface proteins implicated inBiochemistry and biophysics reports · 2024Article
- Novel Ion Channel Genes in Malaria Parasites.Genes · 2024Review
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- A nuclear redox sensor modulates gene activation andProceedings of the National Academy of Sciences of the United States of America · 2022Article
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- Expansion Microscopy RevealsMicroorganisms · 2021Article
- CRISPR/Cas9-engineered inducible gametocyte producer lines as a valuable tool for Plasmodium falciparum malaria transmission research.Nature communications · 2021Article
- The PfAP2-G2 transcription factor is a critical regulator of gametocyte maturation.Molecular microbiology · 2021Article
- Article
- Nuclear Pore Complex Components in the Malaria Parasite Plasmodium berghei.Scientific reports · 2018Article
- Independent regulation of Plasmodium falciparum rif gene promoters.Scientific reports · 2018Article
- Characterization of a nuclear pore protein sheds light on the roles and composition of the Toxoplasma gondii nuclear pore complex.Cellular and molecular life sciences : CMLS · 2017Article
- Specialising the parasite nucleus: Pores, lamins, chromatin, and diversity.PLoS pathogens · 2017Review
- Trans-acting GC-rich non-coding RNA at var expression site modulates gene counting in malaria parasite.Nucleic acids research · 2016Article
- Plasmodium falciparum PfSET7: enzymatic characterization and cellular localization of a novel protein methyltransferase in sporozoite, liver and erythrocytic stage parasites.Scientific reports · 2016Article
- Upstream AUGs and upstream ORFs can regulate the downstream ORF in Plasmodium falciparum.Malaria journal · 2015Article
- Genome-wide profiling of chromosome interactions in Plasmodium falciparum characterizes nuclear architecture and reconfigurations associated with antigenic variation.Molecular microbiology · 2013Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human malaria parasite Plasmodium falciparum modifies the erythrocyte it infects by exporting variant proteins to the host cell surface. The var gene family that codes for a large, variant adhesive surface protein called P. falciparum erythrocyte membrane protein 1 (PfEMP1) plays a particular role in this process, which is linked to pathogenesis and immune evasion. A single member of this gene family is highly transcribed while the other 59 members remain silenced. Importantly, var gene transcription occurs at a spatially restricted, but yet undefined, perinuclear site that is distinct from repressed var gene clusters. To advance our understanding of monoallelic expression, we investigated whether nuclear pores associate with the var gene expression site. To this end, we studied the nuclear pore organization during the asexual blood stage using a specific antibody directed against a subunit of the nuclear pore, P. falciparum Nup116 (PfNup116). Ring and schizont stage parasites showed highly polarized nuclear pore foci, whereas in trophozoite stage nuclear pores redistributed over the entire nuclear surface. Colocalization studies of var transcripts and anti-PfNup116 antibodies showed clear dissociation between nuclear pores and the var gene expression site in ring stage. Similar results were obtained for another differentially transcribed perinuclear gene family, the ribosomal DNA units. Furthermore, we show that in the poised state, the var gene locus is not physically linked to nuclear pores. Our results indicate that P. falciparum does form compartments of high transcriptional activity at the nuclear periphery which are, unlike the case in yeast, devoid of nuclear pores.
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