ArticleJOP : Journal of the pancreas2013
Analysis of endoscopic pancreatic function test (ePFT)-collected pancreatic fluid proteins precipitated via ultracentrifugation.
Article in JOP : Journal of the pancreas, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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Who cites it
3 citing papers in PubMed.
- HBFP: a new repository for human body fluid proteome.Database : the journal of biological databases and curation · 2021Article
- Human body-fluid proteome: quantitative profiling and computational prediction.Briefings in bioinformatics · 2021Review
- Endoscopic Pancreas Fluid Collection: Methods and Relevance for Clinical Care and Translational Science.The American journal of gastroenterology · 2016Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
contextWe have shown previously that trichloroacetic acid precipitation is an effective method of protein extraction from pancreatic fluid for downstream biomarker discovery, compared to other common extraction methods tested.
objectiveWe aim to assess the utility of ultracentrifugation as an alternative method of protein extraction from pancreatic fluid.
designProteins extracted from trichloroacetic acid- and ultracentrifugation-precipitated pancreatic fluid were identified using mass spectrometry techniques (in-gel tryptic digestion followed by liquid chromatography-tandem mass spectrometry; GeLC-MS/MS). Data were analyzed using Proteome Discoverer and Scaffold 3.
settingThis is a proteomic analysis experiment of endoscopically collected fluid in an academic center. PATIENTS: The study population included adult patients referred to the Center for Pancreatic Disease at Brigham and Women's Hospital, Boston, MA, USA for the evaluation of abdominal pain and gastrointestinal symptoms.
interventionsSecretin-stimulated pancreatic fluid was collected as standard of care for the evaluation of abdominal pain and gastrointestinal symptoms.
main outcome measuresWe compared proteins identified via standard trichloroacetic acid precipitation and this alternative ultracentrifugation strategy.
resultsA subset of pancreatic fluid proteins was identified via the ultracentrifugation method. Of these proteins, similar numbers were obtained from fully tryptic or semi-tryptic database searching. Proteins identified in the ultracentrifugation-precipitated samples included previously identified biomarker candidates of chronic pancreatitis.
conclusionsThis alternative ultracentrifugation strategy requires less time and fewer handling procedures than standard trichloroacetic acid precipitation, at the expense of higher sample volume. As such, this method is well suited for targeted assays (i.e., dot blotting or targeted mass spectrometry) if the protein of interest is among those readily identified by ultracentrifugation-promoted precipitation.
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