ArticleBiophysical journal2013
Robustness of self-organizing chemoattractant field arising from precise pulse induction of its breakdown enzyme: a single-cell level analysis of PDE expression in Dictyostelium.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- Many Cells Make Light Work: Self-Generated Gradients OrganizeCold Spring Harbor perspectives in biology · 2026Review
- From microscope to model: rotating signaling dynamics and cluster size in Dictyostelium discoideum aggregates.BMC molecular and cell biology · 2026Article
- Multi-color fluorescence live-cell imaging in Dictyostelium discoideum.Cell structure and function · 2024Article
- Collective signalling drives rapid jumping between cell states.Development (Cambridge, England) · 2023Article
- Deformability and collision-induced reorientation enhance cell topotaxis in dense microenvironments.Biophysical journal · 2023Article
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- A counter-enzyme complex regulates glutamate metabolism in Bacillus subtilis.Nature chemical biology · 2022Article
- Cell dispersal by localized degradation of a chemoattractant.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Proteomic and Transcriptomic Profiling Identifies Early Developmentally Regulated Proteins inCells · 2019Article
- Adenylyl cyclase A mRNA localized at the back of cells is actively translated in live chemotaxingJournal of cell science · 2018Article
- Fold-change detection and scale invariance of cell-cell signaling in social amoeba.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Self-organization of chemoattractant waves in Dictyostelium depends on F-actin and cell-substrate adhesion.Journal of the Royal Society, Interface · 2016Article
- A continuum model of transcriptional bursting.eLife · 2016Article
- Modeling oscillations and spiral waves in Dictyostelium populations.Physical review. E, Statistical, nonlinear, and soft matter physics · 2015Article
- Three-Dimensional Image of Cleavage Bodies in Nuclei Is Configured Using Gas Cluster Ion Beam with Time-of-Flight Secondary Ion Mass Spectrometry.Scientific reports · 2015Article
- Oscillation of cAMP and Ca(2+) in cardiac myocytes: a systems biology approach.The journal of physiological sciences : JPS · 2015Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The oscillation of chemoattractant cyclic AMP (cAMP) in Dictyostelium discoideum is a collective phenomenon that occurs when the basal level of extracellular cAMP exceeds a threshold and invokes cooperative mutual excitation of cAMP synthesis and secretion. For pulses to be relayed from cell to cell repetitively, secreted cAMP must be cleared and brought down to the subthreshold level. One of the main determinants of the oscillatory behavior is thus how much extracellular cAMP is degraded by extracellular phosphodiesterase (PDE). To date, the exact nature of PDE gene regulation remains elusive. Here, we performed live imaging analysis of mRNA transcripts for pdsA--the gene encoding extracellular PDE. Our analysis revealed that pdsA is upregulated during the rising phase of cAMP oscillations. Furthermore, by analyzing isolated cells, we show that expression of pdsA is strictly dependent on the presence of extracellular cAMP. pdsA is induced only at ∼1 nM extracellular cAMP, which is almost identical to the threshold concentration for the cAMP relay response. The observed precise regulation of PDE expression together with degradation of extracellular cAMP by PDE form a dual positive and negative feedback circuit, and model analysis shows that this sets the cAMP level near the threshold concentration for the cAMP relay response for a wide range of adenylyl cyclase activity. The overlap of the thresholds could allow oscillations of chemoattractant cAMP to self-organize at various starving conditions, making its development robust to fluctuations in its environment.
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