ArticleBiophysical journal2013
Aggregation distributions on cells determined by photobleaching image correlation spectroscopy.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells.FEBS open bio · 2026Article
- Polarized Phase-Sensitive Fluorescence-Image Correlation Spectroscopy.Biomolecules · 2026Article
- From Neural Networks to Transformers: Achieving High and Fast Precision in Fluorescence Correlation Spectroscopy Analysis.ACS omega · 2025Article
- Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications.Communications biology · 2023Review
- Evaluation of sted super-resolution image quality by image correlation spectroscopy (QuICS).Scientific reports · 2021Article
- Multicolor fluorescence fluctuation spectroscopy in living cells via spectral detection.eLife · 2021Article
- Fluorescence-based techniques for the detection of the oligomeric status of proteins: implication in amyloidogenic diseases.European biophysics journal : EBJ · 2021Review
- Uptake quantification of gold nanoparticles inside of cancer cells using high order image correlation spectroscopy.Biomedical optics express · 2021Article
- Review
- The architecture of EGFR's basal complexes reveals autoinhibition mechanisms in dimers and oligomers.Nature communications · 2018Article
- Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy.Journal of visualized experiments : JoVE · 2018Article
- Fluorescence-based approaches for monitoring membrane receptor oligomerization.Journal of biosciences · 2018Review
- State-of-the-Art Fluorescence Fluctuation-Based Spectroscopic Techniques for the Study of Protein Aggregation.International journal of molecular sciences · 2018Review
- Probing the intracellular refractive index and molecular interaction of gold nanoparticles in HeLa cells using single particle spectroscopy.International journal of nanomedicine · 2018Article
- Netrin-1-Regulated Distribution of UNC5B and DCC in Live Cells Revealed by TICCS.Biophysical journal · 2016Article
- Spatial Intensity Distribution Analysis Reveals Abnormal Oligomerization of Proteins in Single Cells.Biophysical journal · 2015Article
- SpIDA Surveys the Intricate Web of Macromolecular Oligomerization In Situ.Biophysical journal · 2015Article
- Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs).Chemical reviews · 2014Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The organization of molecules into macromolecular (nanometer scale), supramolecular complexes (submicron-to-micron scale), and within subcellular domains, is an important architectural principle of cellular biology and biochemistry. Determining the precise nature and distribution of complexes within the cellular milieu is a challenging biophysical problem. Time-series analysis of laser scanning confocal microscopy images by image correlation spectroscopy (ICS) or fluctuation moments methods provides information on aggregation, flow, and dynamics of fluorescently tagged macromolecules. All the methods to date require a brightness standard to relate the experimental data to absolute aggregation. In this article, we show that ICS as a function of gradual photobleaching is a sensitive indicator of aggregation distribution on the submicron scale. Specifically, in photobleaching ICS, the extent of nonlinearity of the apparent cluster density as a function of bleaching is related to the size of clusters. The analysis is tested using computer simulations on model aggregate systems and then applied to an experimental determination of Aβ peptide aggregation on nerve cells. The analysis reveals time-dependent increases in Aβ1-42 peptide aggregation. Globally, the datasets could be described by a monomer-dimer-tetramer-hexamer or a monomer-dimer-trimer-pentamer model. The results demonstrate the utility of photobleaching with ICS for determining aggregation states on the supramolecular scale in intact cells without the requirement for a brightness standard.
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