ArticleBiophysical journal2013
An automated live imaging platform for studying merozoite egress-invasion in malaria cultures.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Imaging malaria parasites across scales and time.Journal of microscopy · 2026Review
- Application of optical tweezer technology reveals that PfEBA and PfRH ligands, not PfMSP1, play a central role in Plasmodium falciparum merozoite-erythrocyte attachment.PLoS pathogens · 2024Article
- Plasmodium falciparum formins are essential for invasion and sexual stage development.Communications biology · 2023Article
- Biophysical Tools and Concepts Enable Understanding of Asexual Blood Stage Malaria.Frontiers in cellular and infection microbiology · 2022Review
- Article
- Plug for the parasitophorous duct: a solution of two conundra.Malaria journal · 2020Article
- Stochastic bond dynamics facilitates alignment of malaria parasite at erythrocyte membrane upon invasion.eLife · 2020Article
- Importance of Erythrocyte Deformability for the Alignment of Malaria Parasite upon Invasion.Biophysical journal · 2019Article
- Human Antibodies that Slow Erythrocyte Invasion Potentiate Malaria-Neutralizing Antibodies.Cell · 2019Article
- Endothelial glycocalyx regulates cytoadherence in Plasmodium falciparum malaria.Journal of the Royal Society, Interface · 2018Article
- Rounding precedes rupture and breakdown of vacuolar membranes minutes before malaria parasite egress from erythrocytes.Cellular microbiology · 2018Article
- Evidence against a Role of Elevated Intracellular CaBiophysical journal · 2018Article
- Synergistic malaria vaccine combinations identified by systematic antigen screening.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Article
- Processing of Plasmodium falciparum Merozoite Surface Protein MSP1 Activates a Spectrin-Binding Function Enabling Parasite Egress from RBCs.Cell host & microbe · 2015Article
- Revealing the sequence and resulting cellular morphology of receptor-ligand interactions during Plasmodium falciparum invasion of erythrocytes.PLoS pathogens · 2015Article
- Dynamic morphology and cytoskeletal protein changes during spontaneous inside-out vesiculation of red blood cell membranes.Pflugers Archiv : European journal of physiology · 2014Article
- Quantitation of malaria parasite-erythrocyte cell-cell interactions using optical tweezers.Biophysical journal · 2014Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most cases of severe and fatal malaria are caused by the intraerythrocytic asexual reproduction cycle of Plasmodium falciparum. One of the most intriguing and least understood stages in this cycle is the brief preinvasion period during which dynamic merozoite-red-cell interactions align the merozoite apex in preparation for penetration. Studies of the molecular mechanisms involved in this process face formidable technical challenges, requiring multiple observations of merozoite egress-invasion sequences in live cultures under controlled experimental conditions, using high-resolution microscopy and a variety of fluorescent imaging tools. Here we describe a first successful step in the development of a fully automated, robotic imaging platform to enable such studies. Schizont-enriched live cultures of P. falciparum were set up on an inverted stage microscope with software-controlled motorized functions. By applying a variety of imaging filters and selection criteria, we identified infected red cells that were likely to rupture imminently, and recorded their coordinates. We developed a video-image analysis to detect and automatically record merozoite egress events in 100% of the 40 egress-invasion sequences recorded in this study. We observed a substantial polymorphism of the dynamic condition of pre-egress infected cells, probably reflecting asynchronies in the diversity of confluent processes leading to merozoite release.
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