ArticleBiophysical journal2013
Spatial organization and mechanical properties of the pericellular matrix on chondrocytes.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Cell blebbing novel therapeutic possibilities to counter metastasis.Clinical & experimental metastasis · 2024Review
- Cadherin Expression Is Regulated by Mechanical Phenotypes of Fibroblasts in the Perivascular Matrix.Cells, tissues, organs · 2024Article
- Cumulus expansion is impaired with advanced reproductive age due to loss of matrix integrity and reduced hyaluronan.Aging cell · 2023Article
- Glycosaminoglycans modulate long-range mechanical communication between cells in collagen networks.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Vitamin D regulation of HAS2, hyaluronan synthesis and metabolism in triple negative breast cancer cells.The Journal of steroid biochemistry and molecular biology · 2020Article
- KCells · 2020Review
- Self-regenerating giant hyaluronan polymer brushes.Nature communications · 2019Article
- Hyaluronan Disrupts Cardiomyocyte Organization within 3D Fibrin-Based Hydrogels.Biophysical journal · 2019Article
- The Resting Potential and KFrontiers in physiology · 2018Article
- Single-Molecule Imaging of Proteoglycans in the Pericellular Matrix.Biophysical journal · 2017Article
- Pericellular Brush and Mechanics of Guinea Pig Fibroblast Cells Studied with AFM.Biophysical journal · 2016Article
- Micromechanical Analysis of the Hyaluronan-Rich Matrix Surrounding the Oocyte Reveals a Uniquely Soft and Elastic Composition.Biophysical journal · 2016Article
- The Mechanics of Ovulation Depend on an Incredibly Soft and Sugar-Rich Extracellular Matrix.Biophysical journal · 2016Article
- Cell Surface Access Is Modulated by Tethered Bottlebrush Proteoglycans.Biophysical journal · 2016Article
- Controlled Immobilization Strategies to Probe Short Hyaluronan-Protein Interactions.Scientific reports · 2016Article
- Measuring Local Viscosities near Plasma Membranes of Living Cells with Photonic Force Microscopy.Biophysical journal · 2015Article
- Characterization of a multilayer heparin coating for biomolecule presentation to human mesenchymal stem cell spheroids.Biomaterials science · 2014Article
- Extremely charged and incredibly soft: physical characterization of the pericellular matrix.Biophysical journal · 2013Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A voluminous polymer coat adorns the surface of many eukaryotic cells. Although the pericellular matrix (PCM) often extends several microns from the cell surface, its macromolecular structure remains elusive. This massive cellular organelle negotiates the cell's interaction with surrounding tissue, influencing important processes such as cell adhesion, mitosis, locomotion, molecular sequestration, and mechanotransduction. Investigations of the PCM's architecture and function have been hampered by the difficulty of visualizing this invisible hydrated structure without disrupting its integrity. In this work, we establish several assays to noninvasively measure the ultrastructure of the PCM. Optical force probe assays show that the PCM of rat chondrocyte joint (RCJ-P) cells easily reconfigures around optically manipulated microparticles, allowing the probes to penetrate into rather than compress the matrix. We report distinct changes in forces measured from PCMs treated with exogenous aggrecan, illustrating the assay's potential to probe proteoglycan distribution. Measurements reveal an exponentially increasing osmotic force in the PCM arising from an inherent concentration gradient. With this result, we estimate the variation of the PCM's mesh size (correlation length) to range from ∼100 nm at the surface to 500 nm at its periphery. Quantitative particle exclusion assays confirm this prediction and show that the PCM acts like a sieve. These assays provide a much-needed tool to study PCM ultrastructure and its poorly defined but important role in fundamental cellular processes.
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