ArticleThe EMBO journal1990
Transcription of a nematode trans-spliced leader RNA requires internal elements for both initiation and 3' end-formation.
Article in The EMBO journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Rendering the Intractable More Tractable: Tools from Caenorhabditis elegans Ripe for Import into Parasitic Nematodes.Genetics · 2015Review
- Trans-splicing and operons in C. elegans.WormBook : the online review of C. elegans biology · 2012Review
- Deep small RNA sequencing from the nematode Ascaris reveals conservation, functional diversification, and novel developmental profiles.Genome research · 2011Article
- The nematode eukaryotic translation initiation factor 4E/G complex works with a trans-spliced leader stem-loop to enable efficient translation of trimethylguanosine-capped RNAs.Molecular and cellular biology · 2010Article
- Nematode m7GpppG and m3(2,2,7)GpppG decapping: activities in Ascaris embryos and characterization of C. elegans scavenger DcpS.RNA (New York, N.Y.) · 2004Article
- Unconventional rules of small nuclear RNA transcription and cap modification in trypanosomatids.Gene expression · 2002Review
- Trans-spliced leader addition to mRNAs in a cnidarian.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- trans splicing of polycistronic Caenorhabditis elegans pre-mRNAs: analysis of the SL2 RNA.Molecular and cellular biology · 2000Article
- Transient expression of DNA and RNA in parasitic helminths by using particle bombardment.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Alterations in the conserved SL1 trans-spliced leader of Caenorhabditis elegans demonstrate flexibility in length and sequence requirements in vivo.Molecular and cellular biology · 1999Article
- Transcription termination and 3'-End processing of the spliced leader RNA in kinetoplastids.Molecular and cellular biology · 1999Article
- In vivo function of mutated spliced leader RNAs in Caenorhabditis elegans.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Spliced leader RNA of trypanosomes: in vivo mutational analysis reveals extensive and distinct requirements for trans splicing and cap4 formation.The EMBO journal · 1996Article
- A conserved upstream element is essential for transcription of the Leishmania tarentolae mini-exon gene.The EMBO journal · 1994Article
- Functional analysis of cis-acting DNA elements required for expression of the SL RNA gene in the parasitic protozoan Leishmania amazonensis.Nucleic acids research · 1994Article
- Intramolecular base pairing between the nematode spliced leader and its 5' splice site is not essential for trans-splicing in vitro.The EMBO journal · 1991Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We have used block substitution mutagenesis and in vitro transcription to define sequence elements important for efficient initiation and 3' end-formation of the trans-spliced leader RNA (SL RNA) of the parasitic nematode Ascaris lumbricoides. These experiments indicate that the SL RNA has an unusual promoter structure containing elements which include the 22 nt trans-spliced leader exon itself. Efficient transcription is correlated with the binding of a factor to the 22 nt (SL) sequence; mutations within the SL which abolish transcription lead to a loss in binding of this factor. In addition to internal sequences, synthesis of SL RNA in vitro requires an element centered 50 bases upstream of the cap site. Mutations within this element dramatically affect the level of SL RNA synthesis but do not affect accuracy of initiation. Finally, all of the information required for accurate 3' end-formation of SL RNA lies within the transcribed region. Thus, the arrangement of sequences necessary for the synthesis of SL RNAs does not resemble that of sequences important for the synthesis of vertebrate U snRNAs despite the similarities between SL RNAs and U snRNAs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.