ArticleThe EMBO journal1990
Positive and negative control of translation by the leader sequence of cauliflower mosaic virus pregenomic 35S RNA.
Article in The EMBO journal, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 90 citations in OpenAlex.
- Nuclear export of plant pararetrovirus mRNAs involves the TREX complex, two viral proteins and the highly structured 5' leader region.Nucleic acids research · 2021Article
- Viral Short ORFs and Their Possible Functions.Proteomics · 2018Review
- Ribosome Shunting, Polycistronic Translation, and Evasion of Antiviral Defenses in Plant Pararetroviruses and Beyond.Frontiers in microbiology · 2018Review
- Know Your Enemy: Successful Bioinformatic Approaches to Predict Functional RNA Structures in Viral RNAs.Frontiers in microbiology · 2017Review
- Mitogen-activated protein kinase kinase 5 (MKK5)-mediated signalling cascade regulates expression of iron superoxide dismutase gene in Arabidopsis under salinity stress.Journal of experimental botany · 2015Article
- Ribosomal protein S25 dependency reveals a common mechanism for diverse internal ribosome entry sites and ribosome shunting.Molecular and cellular biology · 2013Article
- The large intergenic region of Rice tungro bacilliform virus evolved differentially among geographically distinguished isolates.Virus genes · 2012Article
- Molecular dissection of the prototype foamy virus (PFV) RNA 5'-UTR identifies essential elements of a ribosomal shunt.Nucleic acids research · 2009Article
- Article
- Improvement of the pBI121 plant expression vector by leader replacement with a sequence combining a poly(CAA) and a CT motif.Transgenic research · 2007Article
- Mechanism of ribosome shunting in Rice tungro bacilliform pararetrovirus.RNA (New York, N.Y.) · 2006Article
- The cauliflower mosaic virus 35S promoter extends into the transcribed region.Journal of virology · 2004Article
- Cestrum yellow leaf curling virus (CmYLCV) promoter: a new strong constitutive promoter for heterologous gene expression in a wide variety of crops.Plant molecular biology · 2003Article
- A promoter derived from taro bacilliform badnavirus drives strong expression in transgenic banana and tobacco plants.Plant cell reports · 2003Article
- Identification of a cis-acting element required for shunt-mediated translational initiation of the Sendai virus Y proteins.Nucleic acids research · 2003Article
- Viral strategies of translation initiation: ribosomal shunt and reinitiation.Progress in nucleic acid research and molecular biology · 2002Review
- Promoters for pregenomic RNA of banana streak badnavirus are active for transgene expression in monocot and dicot plants.Plant molecular biology · 2001Article
- Function of the intercistronic region of BRLF1-BZLF1 bicistronic mRNA in translating the zta protein of Epstein-Barr virus.Journal of virology · 2001Article
- Ribosome shunt is essential for infectivity of cauliflower mosaic virus.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Ribosome shunting in the cauliflower mosaic virus 35S RNA leader is a special case of reinitiation of translation functioning in plant and animal systems.Genes & development · 2000Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied the influence of the 600 nt long leader sequence of cauliflower mosaic virus 35S RNA on downstream translation. Plant protoplasts were transfected with plasmids expressing a CAT reporter gene from a mRNA, containing wild-type or mutant forms of the 35S RNA leader. Deletion analysis revealed the presence of three separate stimulatory sequence regions, S1, S2 and S3. The latter two interact with each other to enhance downstream translation 5- to 10-fold. This enhancement was not observed in protoplasts from a non-host plant. In the absence of either S2 or S3, the region I2, located in between, exerts an inhibitory effect on downstream translation, probably due to the presence of short open reading frames. Expression of a reporter gene inserted into I2 increases 2-fold upon deletion of either S2 or S3. We propose that mRNA regions S2 and S3 form a complex with cellular factors that allows scanning ribosomes to bypass region I2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.