ArticleThe journal of physical chemistry. B2013
Compensating effects of urea and trimethylamine-N-oxide on the heteroassociation of α-chymotrypsin and soybean trypsin inhibitor.
Article in The journal of physical chemistry. B, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
3 citing papers in PubMed.
- Measuring the affinity of protein-protein interactions on a single-molecule level by mass photometry.Analytical biochemistry · 2020Article
- TMAO-Protein Preferential Interaction Profile Determines TMAO's Conditional In Vivo Compatibility.Biophysical journal · 2016Article
- Quantitative characterization of the compensating effects of trimethylamine-N-oxide and guanidine hydrochloride on the dissociation of human cyanmethmoglobin.The journal of physical chemistry. B · 2013Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
An assay for the determination of the equilibrium constant for heteroassociation of α-chymotrypsin and soybean trypsin inhibitor via fluorescence depolarization is described. Results obtained at neutral pH in saline buffer were consistent with prior determinations via sedimentation equilibrium and static light scattering. The dependence of the association equilibrium constant upon the concentrations of urea and trimethylamine-N-oxide (TMAO) added individually and in mixtures was determined at several temperatures. It was found that subdenaturing concentrations of urea decrease the extent of heteroassociation and that added TMAO increases the extent of heteroassociation. The effects of both cosolutes in mixtures upon the equilibrium heteroassociation of α-chymotrypsin and soybean trypsin inhibitor appear to be additive. A thermodynamic analysis of the combined results is presented.
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