ArticleThe Journal of biological chemistry2013
Ras/cAMP-dependent protein kinase (PKA) regulates multiple aspects of cellular events by phosphorylating the Whi3 cell cycle regulator in budding yeast.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- RNA-specific local translation is patterned by condensates for multinucleate cell growth.Nature cell biology · 2026Article
- Article
- Hyperactive Ras disrupts cell size control and a key step in cell cycle entry in budding yeast.Genetics · 2023Article
- TORC1 and PKA activity towards ribosome biogenesis oscillates in synchrony with the budding yeast cell cycle.Journal of cell science · 2022Article
- Transcriptomic and metabolomic analysis reveals genes related to stress tolerance in high gravity brewing.World journal of microbiology & biotechnology · 2022Article
- Phosphoregulation provides specificity to biomolecular condensates in the cell cycle and cell polarity.The Journal of cell biology · 2020Article
- Article
- Review
- Similar environments but diverse fates: Responses of budding yeast to nutrient deprivation.Microbial cell (Graz, Austria) · 2016Article
- Nutrient Control of Yeast Gametogenesis Is Mediated by TORC1, PKA and Energy Availability.PLoS genetics · 2016Article
- Experimental testing of a new integrated model of the budding yeast Start transition.Molecular biology of the cell · 2015Article
- Effects of the yeast RNA-binding protein Whi3 on the half-life and abundance of CLN3 mRNA and other targets.PloS one · 2013Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Start/G1 phase in the cell cycle is an important period during which cells determine their developmental fate, onset of mitotic progression, or the switch to developmental stages in response to both external and internal signals. In the budding yeast Saccharomyces cerevisiae, Whi3, a negative regulator of the G1 cyclins, has been identified as a positive regulator of cell size control and is involved in the regulation of Start. However, the regulatory pathway of Whi3 governing the response to multiple signals remains largely unknown. Here, we show that Whi3 is phosphorylated by the Ras/cAMP-dependent protein kinase (PKA) and that phosphorylation of Ser-568 in Whi3 by PKA plays an inhibitory role in Whi3 function. Phosphorylation of Whi3 by PKA led to its decreased interaction with CLN3 G1 cyclin mRNA and was required for the promotion of G1/S progression. Furthermore, we demonstrate that the phosphomimetic S568D mutation of Whi3 prevented the developmental fate switch to sporulation or invasive growth. Thus, PKA modulated the function of Whi3 by phosphorylation, thus implicating PKA-mediated modulation of Whi3 in multiple cellular events.
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