ArticleMycopathologia2013
Use of rolling circle amplification to rapidly identify species of Cladophialophora potentially causing human infection.
Article in Mycopathologia, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Article
- Isolation and molecular characterization of clinical and environmental dematiaceous fungi and relatives from Iran.Current medical mycology · 2021Article
- Environmental prospecting of black yeast-like agents of human disease using culture-independent methodology.Scientific reports · 2020Article
- New Molecular Markers Distinguishing Fonsecaea Agents of Chromoblastomycosis.Mycopathologia · 2019Article
- CNS Infections Caused by Brown-Black Fungi.Journal of fungi (Basel, Switzerland) · 2019Review
- Use of Padlock Probes and Rolling Circle Amplification (RCA) for Rapid Identification of Trichophyton Species, Related to Human and Animal Disorder.Jundishapur journal of microbiology · 2015Article
- Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification.Frontiers in microbiology · 2015Article
- Rapid identification of black grain eumycetoma causative agents using rolling circle amplification.PLoS neglected tropical diseases · 2014Article
- Veronaea botryosa: molecular identification with amplified fragment length polymorphism (AFLP) and in vitro antifungal susceptibility.Mycopathologia · 2013Article
- The bright future of darkness--the rising power of black fungi: black yeasts, microcolonial fungi, and their relatives.Mycopathologia · 2013Article
- Severe disseminated phaeohyphomycosis in an immunocompetent patient caused by Veronaea botryosa.Mycopathologia · 2013Article
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Authors and funding
8 authors.
Funding
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Abstract
The genus Cladophialophora comprises etiologic agents of disease in immunocompetent patients, ranging from mild cutaneous colonization to cerebral encephalitis, in addition to saprobic species. Due to the high degree of phenotypic similarity between closely related species of the genus, identification problems are imminent. In the present study, we described rapid and sensitive rolling circle amplification (RCA) method based on species-specific padlock probes targeted for the internal transcribed spacer regions of rDNA. ITS regions of 12 Cladophialophora species were sequenced, and subsequently, 10 specific padlock probes were designed for the detection of single nucleotide polymorphisms. The majority of circularizable padlock probes were designed based on single nucleotide polymorphisms (SNPs), while for C. bantiana, C. immunda and C. devriesii were characterized by two or more nucleotides. Individual species-specific probes correctly identified in all ten Cladophialophora species correctly by visualization on 1.2 % agarose gels used to verify specificity of probe-template binding; no cross-reactivity was observed. Simplicity, sensitivity, robustness and low costs provide RCA a distinct place among isothermal techniques for DNA diagnostics. However, restriction and specificity and sensitivity should be lowered and increased, respectively, to be useful for a wide variety of clinical applications.
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