ArticleScience (New York, N.Y.)2013
Cell death from antibiotics without the involvement of reactive oxygen species.
Article in Science (New York, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 298 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
298 citing papers in PubMed, 583 citations in OpenAlex.
- Clarithromycin, trimethoprim, and penicillin and oxidative nucleic acid modifications in humans: randomised, controlled trials.British journal of clinical pharmacology · 2017Trial
- Dual roles of genes required for intrinsic resistance to clarithromycin in evasion of killing by serum complement inInfection and immunity · 2026Article
- The Synergy Between a Silver-Ruthenium Antimicrobial and Aminoglycosides Is Based on Severe Macromolecular Damage.Molecular microbiology · 2026Article
- Antibiotic-Induced Genotoxicity: Molecular Mechanisms, Cytogenetic Damage, and Implications for Human Health.International journal of molecular sciences · 2026Review
- The Effect of Hydrogen Sulfide and Reactive Sulfur Species on Bacterial Virulence and Antibiotic Sensitivity.International journal of molecular sciences · 2026Review
- Role of ClpX and MsrA/B in the Oxidative and Cell Wall Stress Response in Bacillus anthracis Sterne.MicrobiologyOpen · 2026Article
- Reactive oxygen detoxification contributes toeLife · 2026Article
- Implications of bacterial exposure to reactive oxygen and chlorine species.Infection and immunity · 2026Review
- Ferrous lactate-loaded hydrogels induce iron-dependent non-canonical ferroptosis inMaterials today. Bio · 2026Article
- Syntheses, DNA interactions, and photodynamic antimicrobial properties of near-infrared indolium carbocyanine dyes.RSC advances · 2026Article
- Protective role of VitC, Glutathione and Sodium Pyruvate against antibiotic-induced oxidative damage in Escherichia coli.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Antibiotic Mechanisms and Resistance: Molecular Insights and Therapeutic Strategies.Antibiotics (Basel, Switzerland) · 2026Review
- Persistent Gaps in the Ultimate Mechanisms of Antimicrobial-Induced Bacterial Killing.Antibiotics (Basel, Switzerland) · 2026Review
- Article
- Global response to antibiotic exposure reveals a critical role for nucleotide metabolism in high-level β-lactam tolerance.npj antimicrobials and resistance · 2026Article
- The Synergy between a Silver-Ruthenium Antimicrobial and aminoglycosides is based on severe macromolecular damage.bioRxiv : the preprint server for biology · 2026Article
- Article
- Xtr-mediated regulation of 4Fe-4S binding protein gene confers resistance to oxidative and antibiotic stress inFrontiers in microbiology · 2026Article
- Identification of host genetic factors modulating β-lactam resistance inEmerging microbes & infections · 2025Article
- Fluorescein-based dyes are not valid reporters of oxidative stress in bacteria, and conclusions based on their use must be reconsidered.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
238 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Recent observations have suggested that classic antibiotics kill bacteria by stimulating the formation of reactive oxygen species (ROS). If true, this notion might guide new strategies to improve antibiotic efficacy. In this study, the model was directly tested. Contrary to the hypothesis, antibiotic treatment did not accelerate the formation of hydrogen peroxide in Escherichia coli and did not elevate intracellular free iron, an essential reactant for the production of lethal damage. Lethality persisted in the absence of oxygen, and DNA repair mutants were not hypersensitive, undermining the idea that toxicity arose from oxidative DNA lesions. We conclude that these antibiotic exposures did not produce ROS and that lethality more likely resulted from the direct inhibition of cell-wall assembly, protein synthesis, and DNA replication.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.