ArticleNucleic acids research2013
Harnessing mutagenic homologous recombination for targeted mutagenesis in vivo by TaGTEAM.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 57 citations in OpenAlex.
- Harnessing the central dogma: Enzyme-driven gene diversification strategies for in cellulo continuous directed evolution.Fundamental research · 2026Review
- RNA-Based Communication in Heterogeneous Populations of Cell Mimics.ACS synthetic biology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Nickase fidelity drives EvolvR-mediated diversification in mammalian cells.Nature communications · 2025Article
- A meiotic driver hijacks an epigenetic reader to disrupt mitosis in noncarrier offspring.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The Pseudomonas aeruginosa Resistome: Permanent and Transient Antibiotic Resistance, an Overview.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Evolution and synthetic biology.Current opinion in microbiology · 2023Review
- A Rapid Antibody Enhancement Platform inACS synthetic biology · 2023Article
- Translating eco-evolutionary biology into therapy to tackle antibiotic resistance.Nature reviews. Microbiology · 2023Review
- A Cas3-base editing tool for targetable in vivo mutagenesis.Nature communications · 2023Article
- In vivo hypermutation and continuous evolution.Nature reviews. Methods primers · 2022Article
- Microfluidic Production of Porous Polymer Cell-Mimics Capable of Gene Expression.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Systems for in vivo hypermutation: a quest for scale and depth in directed evolution.Current opinion in chemical biology · 2021Review
- Targeted mutagenesis of multiple chromosomal regions in microbes.Current opinion in microbiology · 2020Review
- Automated Continuous Evolution of ProteinsACS synthetic biology · 2020Article
- The developing toolkit of continuous directed evolution.Nature chemical biology · 2020Review
- Article
- Tools and systems for evolutionary engineering of biomolecules and microorganisms.Journal of industrial microbiology & biotechnology · 2019Review
- In vivo continuous evolution of metabolic pathways for chemical production.Microbial cell factories · 2019Review
- Probing pathways of adaptation with continuous evolution.Current opinion in systems biology · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A major hurdle to evolutionary engineering approaches for multigenic phenotypes is the ability to simultaneously modify multiple genes rapidly and selectively. Here, we describe a method for in vivo-targeted mutagenesis in yeast, targeting glycosylases to embedded arrays for mutagenesis (TaGTEAM). By fusing the yeast 3-methyladenine DNA glycosylase MAG1 to a tetR DNA-binding domain, we are able to elevate mutation rates >800 fold in a specific ∼20-kb region of the genome or on a plasmid that contains an array of tetO sites. A wide spectrum of transitions, transversions and single base deletions are observed. We provide evidence that TaGTEAM generated point mutations occur through error-prone homologous recombination (HR) and depend on resectioning and the error-prone polymerase Pol ζ. We show that HR is error-prone in this context because of DNA damage checkpoint activation and base pair lesions and use this knowledge to shift the primary mutagenic outcome of targeted endonuclease breaks from HR-independent rearrangements to HR-dependent point mutations. The ability to switch repair in this way opens up the possibility of using targeted endonucleases in diverse organisms for in vivo-targeted mutagenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.