ArticleInternational journal of medical sciences2013
Improved culture conditions for the growth and detection of Borrelia from human serum.
Article in International journal of medical sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Accuracy of Diagnostic Tests for Lyme Disease in Humans, A Systematic Review and Meta-Analysis of North American Research.PloS one · 2016Pooled it
- Presence of Borrelia Spirochetes in White Stork (Animals : an open access journal from MDPI · 2024Article
- Evidence for the presence of Borrelia burgdorferi in invasive breast cancer tissues.European journal of microbiology & immunology · 2024Article
- Developing a Prospective Gestational Lyme Disease Study.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A Comparative Study of Body Lice and Bed Bugs Reveals Factors Potentially Involved in Differential Vector Competence for the Relapsing Fever Spirochete Borrelia recurrentis.Infection and immunity · 2022Article
- Borrelia burgdorferi Co-Localizing with Amyloid Markers in Alzheimer's Disease Brain Tissues.Journal of Alzheimer's disease : JAD · 2022Article
- Case Report: The First Borrelia yangtzensis Infection in a Human in Korea.The American journal of tropical medicine and hygiene · 2021Article
- First report of Borrelia burgdorferi sensu stricto detection in a commune genospecies in Apodemus agrarius in Gwangju, South Korea.Scientific reports · 2021Article
- Laboratory Diagnosis of Lyme Borreliosis.Clinical microbiology reviews · 2021Review
- Article
- First identification of Anaplasma phagocytophilum in both a biting tick Ixodes nipponensis and a patient in Korea: a case report.BMC infectious diseases · 2020Article
- Article
- Detection and Transstadial Passage ofHealthcare (Basel, Switzerland) · 2019Article
- Case Report: Scrub Typhus and Q Fever Coinfection.The American journal of tropical medicine and hygiene · 2019Article
- Article
- Recent strategies for the diagnosis of early Lyme disease.Science progress · 2018Review
- Far-Reaching Dispersal ofHealthcare (Basel, Switzerland) · 2018Article
- Persistent Borrelia Infection in Patients with Ongoing Symptoms of Lyme Disease.Healthcare (Basel, Switzerland) · 2018Article
- Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR.Journal of visualized experiments : JoVE · 2018Article
- Better drugs for Lyme disease: focus on the spirochete.Infection and drug resistance · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this report we present a method to cultivate Borrelia spirochetes from human serum samples with high efficiency. This method incorporates improved sample collection, optimization of culture media and use of matrix protein. The method was first optimized utilizing Borrelia laboratory strains, and later by demonstrating growth of Borrelia from sera from fifty seropositive Lyme disease patients followed by another cohort of 72 Lyme disease patients, all of whom satisfied the strict CDC surveillance case definition for Lyme disease. The procedure resulted in positive cultures in 47% at 6 days and 94% at week 16. Negative controls included 48 cases. The positive identification of Borrelia was performed by immunostaining, PCR, and direct DNA sequencing.
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Registered trials
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