Evidence map›Paper›PMID 23470775›Full record

ArticleNeuroscience2013

Irisin-immunoreactivity in neural and non-neural cells of the rodent.

S L Dun, R-M Lyu, Y-H Chen, J-K Chang, J J Luo, N J Dun

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
109citing papers in PubMed, 3 pooled it
9.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

109 citing papers in PubMed, 3 syntheses or guidelines pooled it, 227 citations in OpenAlex.

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  7. An overview of immunohistochemistry: Evidence and personal experience.The Journal of international medical research · 2026
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49 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

S L DunDepartment of Pharmacology, Temple University School of Medicine, Philadelphia, PA 19140, USA. ndun@temple.edu
R-M Lyu
Y-H Chen
J-K Chang
J J Luo
N J Dun
Temple University · USAcceleron Pharma (United States) · US

Funding

ACTIVITY OF BRAINSTEM NEURONSR01HL051314 · NHLBI · UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS · PI DUN, NAE J · 1995 to 2010
$2.3M
NHLBI NIH HHS HL51314NHLBI NIH HHS R01 HL051314
6 · The paper itself

Abstract

Irisin is a recently identified myokine secreted from the muscle in response to exercise. In the rats and mice, immunohistochemical studies with an antiserum against irisin peptide fragment (42-112), revealed that irisin-immunoreactivity (irIRN) was detected in three types of cells; namely, skeletal muscle cells, cardiomyocytes, and Purkinje cells of the cerebellum. Tissue sections processed with irisin antiserum pre-absorbed with the irisin peptide (42-112) (1 μg/ml) showed no immunoreactivity. Cerebellar Purkinje cells were also immunolabeled with an antiserum against fibronectin type II domain containing 5 (FNDC5), the precursor protein of irisin. Double-labeling of cerebellar sections with irisin antiserum and glutamate decarboxylase (GAD) antibody showed that nearly all irIRN Purkinje cells were GAD-positive. Injection of the fluorescence tracer Fluorogold into the vestibular nucleus of the rat medulla retrogradely labeled a population of Purkinje cells, some of which were also irIRN. Our results provide the first evidence of expression of irIRN in the rodent skeletal and cardiac muscle, and in the brain where it is present in GAD-positive Purkinje cells of the cerebellum. Our findings together with reports by others led us to hypothesize a novel neural pathway, which originates from cerebellum Purkinje cells, via several intermediary synapses in the medulla and spinal cord, and regulates adipocyte metabolism.

Indexed as

AnimalsBrainCell CountFibronectinsGlutamate DecarboxylaseMaleMiceMice, Inbred ICRMyocardiumNeural PathwaysNeurogliaNeuronsRatsRats, Sprague-DawleyStilbamidines2-hydroxy-4,4'-diamidinostilbene, methanesulfonate saltFibronectinsFNDC5 protein, mouseGlutamate DecarboxylaseStilbamidines

Identifiers

PMID23470775
PMCPMC3637839
OpenAlexW1967155265

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.