ArticlePloS one2013
Catalytic and functional roles of conserved amino acids in the SET domain of the S. cerevisiae lysine methyltransferase Set1.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Prediction of the most deleterious non-synonymous SNPs in the human IL1B gene: evidence from bioinformatics analyses.BMC genomic data · 2024Article
- SETDB1 as a cancer target: challenges and perspectives in drug design.RSC medicinal chemistry · 2024Review
- Unraveling the function and structure impact of deleterious missense SNPs in the human OX1R receptor by computational analysis.Scientific reports · 2024Article
- In silico screening of non-synonymous SNPs in human TUFT1 gene.Journal, genetic engineering & biotechnology · 2023Article
- A CpG island-encoded mechanism protects genes from premature transcription termination.Nature communications · 2023Article
- Histone 3 lysine 4 monomethylation supports activation of transcription in S. cerevisiae during nutrient stress.Current genetics · 2022Article
- Loss of SET1/COMPASS methyltransferase activity reduces lifespan and fertility inLife science alliance · 2022Article
- Exploring the Structural and Functional Effects of Nonsynonymous SNPs in the Human Serotonin Transporter Gene ThroughBioinformatics and biology insights · 2022Article
- In-silico analysis reveals druggable single nucleotide polymorphisms in angiotensin 1 converting enzyme involved in the onset of blood pressure.BMC research notes · 2021Article
- Distinct requirements for the COMPASS core subunits Set1, Swd1, and Swd3 during meiosis in the budding yeast Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2021Article
- Adaptive selection in the evolution of programmed cell death-1 and its ligands in vertebrates.Aging · 2020Article
- Prediction of Deleterious Non-synonymous SNPs of Human STK11 Gene by Combining Algorithms, Molecular Docking, and Molecular Dynamics Simulation.Scientific reports · 2019Article
- The Transcription Factor THO Promotes Transcription Initiation and Elongation by RNA Polymerase I.The Journal of biological chemistry · 2016Article
- In silico Analysis Revealed High-risk Single Nucleotide Polymorphisms in Human Pentraxin-3 Gene and their Impact on Innate Immune Response against Microbial Pathogens.Frontiers in microbiology · 2016Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
In S. cerevisiae, the lysine methyltransferase Set1 is a member of the multiprotein complex COMPASS. Set1 catalyzes mono-, di- and trimethylation of the fourth residue, lysine 4, of histone H3 using methyl groups from S-adenosylmethionine, and requires a subset of COMPASS proteins for this activity. The methylation activity of COMPASS regulates gene expression and chromosome segregation in vivo. To improve understanding of the catalytic mechanism of Set1, single amino acid substitutions were made within the SET domain. These Set1 mutants were evaluated in vivo by determining the levels of K4-methylated H3, assaying the strength of gene silencing at the rDNA and using a genetic assessment of kinetochore function as a proxy for defects in Dam1 methylation. The findings indicate that no single conserved active site base is required for H3K4 methylation by Set1. Instead, our data suggest that a number of aromatic residues in the SET domain contribute to the formation of an active site that facilitates substrate binding and dictates product specificity. Further, the results suggest that the attributes of Set1 required for trimethylation of histone H3 are those required for Pol II gene silencing at the rDNA and kinetochore function.
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